摘要
MicroRNA(miRNA)是一类广泛存在于真核生物中的具有转录后水平调控功能的内源非编码小分子RNA。在植物中,miRNA通过对靶基因的剪切或沉默来实现对植物生命活动的调控,它是基因表达调控网络的重要组成部分。miR165/166(miR166)是陆生植物中最为古老的MIRNA家族之一,它通过对3型同源异域型-亮氨酸拉链(HD-ZIP III)等靶标的调控,在植物的众多发育时期起着关键的调控作用。本文分析了MIR166基因在陆生植物中的进化关系,并对MIR166在基部陆生植物小立碗藓(Physcomitrella patens)中的复制及进化进行了研究。此外,HD-ZIP III蛋白是植物中重要的一类转录因子,miR166对HD-ZIP III基因的调控作用在陆地植物保守的存在,本文对HD-ZIP III基因和miR166在进化中的相互作用进行了初步的探讨。
MicroRNA （miRNA） is a class of endogenous non-coding small RNAs with important post-transcriptional regulatory roles in eukaryotes. Plant miRNAs play important roles in the post-transcriptional regulatory network through mediating cleavage or silencing of target mRNAs. As one of the ancient MIRNA family, miR165/166 （miR166） is a key regulator in land plants. In this study, we analyzed the diversity and molecular evolution of MIR166 genes in land plants, and identified the replication and evolution of MIR166 genes in Physcomitrella patens. Homeodomain Leucinezipper of class Ⅲ （ HD-ZIP Ⅲ） proteins are important transcription factors in plants, HD-ZIP Ⅲ genes are conserved targeting of miR166 in angiosperms, gymnosperms, ferns, and mosses. Here, a preliminary study was conducted to make clear the relationships between HD-ZIP Ⅲ genes and miR166 during the evolutionary process.
出处
《植物分类与资源学报》
CAS
CSCD
北大核心
2014年第3期331-341,共11页
Plant Diversity
基金
The NSFC-Yunnan province joint foundation (U1136603)
the Knowledge Innovation Project of the Chinese Academy of Sciences (KSCX2-YW-N-067)
the National Natural Science Foundation of China (30990244)
Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry and the Young Academic and Technical Leader Raising Foundation of Yunnan Province(2008PY065)
the Western Light Talent Culture Project of the Chinese Academy of Sciences(2010312D11035)
the Yunnan Provincial Government through an innovation team programme
