摘要
以螺旋藻多糖为研究对象,探讨其体内抗H22肿瘤作用及机理。采用双酶方法提取螺旋藻多糖。小鼠腋下接种H22肝癌细胞建立动物模型,以1 000 mg/kg/d灌胃给药。通过瘤重及抑瘤率测定、胸腺指数(TI)及脾脏指数(SI)测定、脾细胞增殖能力测定、NK细胞杀伤活性测定、吞噬细胞吞噬能力测定、小鼠血清TNF-α和IFN-γ测定等研究其抗H22肿瘤作用和机理。给药组抑瘤率达74.53%,提高了胸腺、脾脏指数、NK细胞杀伤活性及巨噬细胞的吞噬能力,增加了血清中TNF-α和IFN-γ的含量,小鼠免疫系统得到有效保护,加强了抗H22肿瘤作用。螺旋藻多糖抗H22肿瘤效果明显,在保护免疫系统及提高免疫力方面起到了很好的作用。
To study the antitumor activity in vivo of Spirulina polysaccharide. Spirulina polysaccharide was extracted with double enzyme hydrolysis. H22 tumor bearing mice were established entity and treated by gavage with 1 000 mg/kg/d of the polysaccharide. The tumor weight, tumor inhibition rate, thymus index and Spleen index, lymphocyte proliferation, kill rate of NK cells, detection of phagocytic, the concentrations of TNF-αand IFN-γin mice blood serum were measured to study antitumor function in vivo. The tumor inhibition rate was 74.53%, The thymus index and Spleen index, lymphocyte proliferation, kill rate of NK cells were raised, and the concentrations of TNF-αand IFN-γ in mice blood serum were increased. The Spirulina polysaccharide had played an important role in protecting the immune system and promoting the immune function of tumor bearing mice.
出处
《食品研究与开发》
CAS
北大核心
2014年第5期120-123,共4页
Food Research and Development
基金
河北联合大学科学研究基金项目资助(Z201313)
关键词
螺旋藻多糖
抗肿瘤
免疫
Spirulina polysaccharide
antitumor
immunity
