摘要
本试验旨在研究富硒益生菌对罗非鱼幼鱼生长及抗氧化水平的影响。试验选取600尾罗非鱼幼鱼，随机分为5组，每组设3个重复，每个重复随机放养初始体重为（12.40±0.13） g的罗非鱼幼鱼40尾。其中，对照组饲喂硒含量为0.257 mg/kg的基础饲料，其他4组分别饲喂在基础饲料中添加由富硒酵母菌、酵母菌、乳酸菌组成的富硒益生菌的试验饲料。4种试验饲料中富硒酵母菌的添加量折算为硒添加量分别为0（A组）、0.4（B组）、0.6（C组）、0.8 mg/kg（D组），乳酸菌的添加量均为3.04×10^5 CFU/g，并通过调节酵母菌添加量使4种试验饲料中添加的总活菌数相同。试验期为70 d。结果表明：D组和C组分别在第50～60天和第60～70天阶段表现出最高的特定生长率，并均显著高于对照组（P＜0.05）。 C组血清谷胱甘肽过氧化物酶（ GPx）活性在第30天和第50天均显著高于A组（P＜0.05）；对照组血清GPx活性在第60天显著低于其他各组（P＜0.05）。在第30天，血清过氧化氢酶（CAT）活性以C组最高、A组最低，各组间均差异显著（P＜0.05）；对照组血清CAT活性在第60天显著低于A组、B组和C组（P＜0.05）。 C组血清超氧化物歧化酶（SOD）活性在第30天和第50天显著高于其他各组（P＜0.05）。各组罗非鱼的肝脏谷胱甘肽过氧化物酶1（GPx1） mRNA相对表达量均随饲养天数的延长呈现出上升态势，至第50天达到最高值后开始呈下降的趋势。在第50天，A组和C组的肝脏GPx1 mRNA的相对表达量显著高于其他各组（P＜0.05），且以C组的值最高。由上述结果可知，在含硒0.257 mg/kg的基础饲料中添加0.6 mg/kg的富硒益生菌（以硒计）可促进罗非鱼幼鱼（体重12～55 g/尾）的生长，提高其抗氧化水平。
This experiment was conducted to study the effects of selenium-enriched probiotics on growth and antioxidant level of juvenile tilapia （ Oreochromis niloticus） .Six hundred juvenile tilapia with an average initial body weight of （12.40±0.13） g were randomly chosen and distributed into 5 groups with 3 replicates in each group and 40 fish in each replicate.The fish in control group were fed a basal diet containing 0.257 mg/kg selenium, and the fish in other four groups were fed experimental diets supplemented with selenium-enriched proiotics.The selenium-enriched probiotics were composed by selenium-enriched yeast, yeast and lactic acid bacteria with different proportions.The supplemental levels of selenium-enriched yeast converted into selenium supplemental levels were 0 （ group A） , 0.4 （ group B） , 0.6 （ group C） and 0.8 mg/kg （ group D） , respectively, while the supplemental levels of lactic acid bacteria were all 3.04×10^5 CFU/g, and the supplemental levels of living bacteria were the same by adjusting yeast supplemental level in the 4 experimental diets.The experiment lasted for 70 days.The results showed as follows： the highest of special growth rate in the stages of days 50 to 60 and days 60 to 70 was found in groups D and C, respectively, and was significantly higher than that in control group （ P〈0.05） .The activity of serum glutathione peroxidase （ GPx） on day 30 and day 50 in group C was significantly higher than that in group A （P〈0.05）, and the activity of serum GPx on day 60 in control group was significantly lower than that in other groups （ P〈0.05） .On day 30, groups C and A had the highest and the lowest serum catalase （ CAT） activity, respectively, and there was significant difference in serum CAT activity among all groups （ P〈0.05） .The activity of serum CAT on day 60 in control group was significantly lower than that in groups A, B and C （ P〈0.05 ） .The activity of serum superoxide dismutase （ SOD） on day 30 and day 50 in group C was significantly higher than that in other groups （ P〈0.05） .The relative expression level of liver glutathione peroxidase 1 （ GPx1） mRNA in each group was firstly increased with feeding time extending; subsequently, it was decreased after reached the highest value on day 50.The relative expression level of liver GPx1 mRNA in groups A and C was significantly higher than that in other groups （ P〈0.05） , and group C had the highest value.In conclusion, the supplementation of 0.6 mg/kg seleniumsnriched probiotics （ counted with selenium） in the basal diet with 0.257 mg/kg selenium can improve the growth and antioxidant level of juvenile tilapia with the body weight of 12 to 55 g per fish.
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第5期1229-1237,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
广州市农科科技攻关项目(GZCQC1202FG04002-01)
广东省海洋渔业科技推广专项(A201001D05
A201201C01)
广州市科技计划项目(201300000064)
