摘要
目的凉血化瘀法对肾组织nephrin及podocin的影响目前研究报道较少。文中通过观察丹芍颗粒Ⅲ对紫癜性肾炎(Henoch-Schnlein purpura nephritis,HSPN)大鼠血尿、蛋白尿和肾组织nephrin及podocin的影响,探讨凉血化瘀法对HSPN大鼠的治疗作用及其机制。方法将21只SD雄性大鼠按随机数字表法分为空白组、模型组、丹芍组,每组7只。模型组和丹芍组建立HSPN模型,丹芍组给予丹芍颗粒Ⅲ治疗。应用尿沉渣计数法检测尿红细胞个数,BCA法检测24 h尿蛋白定量;光镜下观察肾小球系膜变化;采用间接免疫荧光法检测肾小球nephrin及podocin的表达及分布;采用RT-PCR技术检测肾组织nephrin mRNA和podocin mRNA的表达。结果①模型组镜下红细胞计数显著高于空白组(26.5个/高倍视野vs0.3个/高倍视野),24 h尿蛋白定量亦显著高于空白组[(2.214±1.090)g/24 h vs(0.624±0.354)g/24 h],差异有统计学意义(P<0.01),病理结果提示模型组肾组织存在异常改变,说明造模有效;②丹芍组大鼠的尿红细胞计数较模型组明显减少(0.8个/高倍视野vs 26.5个/高倍视野)、尿蛋白定量亦较模型组明显减少[(1.000±0.651)g/24 h vs(2.214±1.090)g/24 h],差异有统计学意义(P<0.01);③丹芍组大鼠肾组织病理显示炎症较模型组减轻;④丹芍组大鼠肾小球nephrin表达量较模型组升高[(65.957±14.414)vs(43.520±0.632)],差异有统计学意义(P<0.01),丹芍组podocin表达较模型组有上升趋势,但差异无统计学意义(P>0.05);丹芍组大鼠肾小球nephrin及podocin分布较模型组有改善;⑤丹芍组大鼠肾组织nephrin mRNA的表达较模型组升高[(0.530±0.089)vs(0.117±0.021)],podocin mRNA的表达较模型组升高[(0.490±0.160)vs(0.033±0.025)],差异有统计学意义(P<0.05)。结论以凉血化瘀为主要作用机制的丹芍颗粒Ⅲ能有效降低HSPN大鼠血尿及蛋白尿水平,改善肾炎症反应。
Objective Few reports are seen about the effects of cooling blood and removing stasis on nephrin and podocin . This study was to evaluate the therapy of cooling blood and removing stasis for Henoch -Sch？nlein purpura nephritis （ HSPN） and its ac-tion mechanisms by observing the effects of Danshao Granular Ⅲ on hematuresis , proteinuria and the expressions of nephridial nephrin and podocin in HSPN rats . Methods Twenty-one SD male rats were e-qually randomized to a blank control , an HSPN model , and a Dan-shao group.At 13 weeks after modeling , the animals in the model group were treated intragastrically with distilled water , while those in the Danshao group with Danshao Granular Ⅲtwice daily for 4 weeks. Then, the urinary red blood cell （ RBC） count was examined , the 〈br〉 24 h urinary protein quantity determined , the glomerular mesangial changes observed under the light microscope , the protein expres-sions and distributions of nephrin and podocin detected by indirect immunofluorescence , and their mRNA expressions determined by re-al-time PCR. Results The urinary RBC count and 24 h urine protein quantity were significantly higher in the HSPN model than in the blank control group （26.5/HP vs 0.3/HP and [2.214 ±1.090]g/24 h vs [0.624 ±0.354]g/24 h, both P＆lt;0.01）.The model rats showed obvious pathological changes in the renal tissue .The urinary RBC count and 24 h urine protein volume were remarkably de-creased in the Danshao group as compared with the models （0.8/HP vs 26.5/HP and [1.000 ±0.651]g/24 h vs [2.214 ±1.090] g/24 h, both P＆lt;0.01）.The pathological changes in the renal tissue of the Danshao group were reduced in comparison with those of the model group.The protein expression of nephrin was higher in the former than in the latter （65.975 ±14.414 vs 43.520 ±0.632, P＆lt;0.01） and so was that of podocin though with no statistically significant difference （P＆gt;0.05）.The distributions of nephrin and podocin were improved after Danshao treatment .The mRNA expressions of nephrin and podocin were markedly higher in the Danshao group than in the HSPN models （0.530 ±0.089 vs 0.117 ±0.021 and 0.490 ±0.160 vs 0.033 ±0.025, P＆lt;0.05）. Conclusion Danshao Granular Ⅲ, with its main action mechanisms of cooling blood and removing stasis , can effectively reduce urinary RBC count and urinary protein quantity and improve the symptoms of HSPN in rats .
出处
《医学研究生学报》
CAS
北大核心
2014年第4期373-377,共5页
Journal of Medical Postgraduates
基金
南京中医药大学中医儿科学重点学科开放课题(EZK2012021)
