摘要
在pH=4.5的HAc-NaAc缓冲介质中,Fe3+能与十二烷基硫酸钠(SDS)和环丙沙星作用,使其荧光值显著减小,据此建立了Fe3+-SDS-环丙沙星体系荧光猝灭法测定微量环丙沙星的新方法。该体系的最大激发、发射波长分别为λex=275 nm,λem=441 nm。环丙沙星在4.0×10-7~3.6×10-6mol/L的浓度范围内与荧光值呈良好的线性关系,R=0.995 8,相对标准偏差为0.76%(c=3.0×10-6mol/L)。
HAc-NaAc Buffer medium of pH = 4. 5,with sodium dodecyl sulfate（SDS）and ciprofloxacin reaction to form a stable complex,and its fluorescence intensity was significantly reduced. Accodingly es-tablished pursuant to Fe3 ＋ -SDS-ciprofloxacin system fluorescence quenching method for the determination of ciprofloxacin. The system maximum excitation and emission wavelength were excitation at 275 nm, emission at 441 nm. Ciprofloxacin 4. 0 × 10 - 7 ~ 3. 6 × 10 - 6 mol/ L concentration in the range of fluores-cence intensity showed a good linear relationship,R = 0. 995 8,the relative standard deviation was 0. 76%（c = 3. 0 × 10 - 6 mol/ L）.
出处
《应用化工》
CAS
CSCD
2014年第5期933-935,共3页
Applied Chemical Industry
基金
国家自然科学基金资助(21201069)
