摘要
目的 观察2μm激光前列腺切除术后犬前列腺部尿道与膀胱颈创伤修复的病理组织学改变,比较前列腺部尿道和膀胱颈修复再上皮化的差别. 方法 2012年4-5月选取健康成年雄性中华田园犬15只,行2μm激光前列腺汽化切除术及部分膀胱颈黏膜切除术,分别于术后3d及1、2、3、4周处死动物,留取前列腺部尿道及膀胱颈标本,HE染色后光镜下观察前列腺部尿道及膀胱颈创伤修复的病理组织学改变,免疫组化染色法检测前列腺部尿道及膀胱颈创面细胞中Ki-67及增殖细胞核抗原（proliferating cell nuclear antigen,PCNA）的表达情况,Masson三色染色并半定量分析检测创面胶原纤维的表达量. 结果 光镜下可见前列腺部尿道创面下残余前列腺上皮增殖、迁移并覆盖创面,完成再上皮化过程,再上皮化于术后1周开始,术后3周基本完成,免疫组化染色检测示增殖的前列腺上皮细胞中Ki-67及PCNA高表达;膀胱颈创面由创缘旁尿路上皮增殖后向创面迁移完成再上皮化过程,再上皮化于术后2周开始,至术后4周仍未完成该过程,膀胱颈创缘旁增生的尿路上皮细胞中Ki-67及PCNA高表达.Masson三色染色并半定量分析检测创面下胶原纤维表达量结果显示,前列腺部尿道创面术后3周和4周均为（＋）,膀胱颈创面分别为（＋＋～＋＋＋）和（＋＋＋）. 结论 前列腺部尿道创面下残余前列腺上皮细胞增殖、迁移并覆盖创面的创伤修复再上皮化方式可能是机体实现由解剖修复到功能修复的最佳修复方式.
Objective To observe the histopathology of the repair in canine prostatic urethra and bladder neck after two micron laser resection of the prostate and bladder neck,and to compare the differences in the re-epithelialization in two parts.Methods From April 2012 to May 2012,two micron laser resection of the prostate and partial bladder neck mucosa were performed in 15 healthy adult male cross bred canines.Canines were sacrificed and wound specimens of prostatic urethra and bladder neck were harvested at 3 days,1,2,3 and 4 weeks after operation,respectively.The histopathology of canine prostatic urethra and bladder neck were observed by HE staining.Immunohistochemical staining was performed to determine expression of Ki-67 and proliferating cell nuclear antigen （PCNA） protein at the wound of prostatic urethra and bladder neck.Masson＇s trichrome staining and semi-quantitative analysis was performed to determine the expression of collagen fibers.Results The histology displayed that re-epithelialization of canine prostatic urethra originated from the mobilization of proliferated epithelial cells from residual prostate tissue under the wound.Re-epithelialization began in 1 week and basically finished in 3 weeks.High expression of Ki-67 and PCNA were noticed in the proliferated prostatic epithelial cells.Re-epithelialization of bladder neck originated from the mobilization of proliferated epithelial cells from the edges of the wound at the bladder neck.Reepithelialization began in 2 weeks and was not complete in 4 weeks.The expression of Ki-67 and PCNA in the proliferated epithelial cells from the edges of the wound were significantly increased.The result by Masson＇s trichrome staining and semi-quantitative analysis showed that the expression of collagen fibers were （＋） in prostatic urethra wounds,and （＋ ＋-＋ ＋ ＋） and （＋ ＋ ＋） in bladder neck wounds at 3 and 4 weeks,respectively.Conclusions Re-epithelialization of prostatic urethra originated from mobilization of proliferated epithelial cells from residual prostate tissue under the wound may be the best way to achieve the anatomic repair and functional recovery.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2014年第5期378-382,共5页
Chinese Journal of Urology
基金
国家自然科学基金（81260119）
贵州省科技厅基金[黔科合人才团队（2012）4013号、黔科合SZ字（2012）3077号、黔科合SY字（2011）3024号、黔科合LS字（2011）020号]
贵州省卫生厅基金（gzwkj2013-1-105）
关键词
良性前列腺增生
创伤修复
再上皮化
Benign prostate hyperplasia
Repair in trauma
Re-epithelialization
