摘要
目的了解大庆地区2009—2011年感染人的轮状病毒流行株的血清型情况。方法采用A组轮状病毒ELISA检测试剂盒对采集来的288例腹泻标本进行轮状病毒阳性鉴定，对鉴定为阳性的腹泻标本经处理后，采用多重一步反转录一聚合酶链反应（RT—PCR）对轮状病毒株的血清型进行鉴定，并通过对阳性标本的轮状病毒VP7基因的核苷酸序列进行分析、比对，以进一步鉴定毒株的血清型。结果对288例标本进行ELISA检测，RV阳性177例；血清型检测共测得G1型73例（41．2％），G2型12例（6．7％），G3型67例（37．9％），另有25例为G1和G3混合型。结论轮状病毒是引起大庆地区婴幼儿病毒性腹泻的主要原因，对成人腹泻影响较小，2009～2011年大庆地区流行的RV以G，和G，型为主。此外，出现了G1和G3混合型，说明病毒血清型之间出现了一定重组变异。
Objective To study the serotype of epidemic strains of human rotavirus infection from 2009 to 2011 in Daqing. Meth- ods We tested the positive for the rotavirus diarrhea samples collected from 288 cases by group A rotavirus ELISA test kit. After the diar- rhea specimens with positive rotavirus was proeessinged,we tested the VP7 （ viral protein 7） serotype of the positive samples through one step multiplex RT - PCR technology, and compared nucleotide sequence of RV VP7 to further identify the serotype strains of RV. Results In 288 cases of ELISA tests specimens,177 cases were the RV positive. Among them,73 （41.2%） eases were recognized as G1,12 （6.7%） were type G2 , 67 （37.9%） were type G3 ,and 25 were admixtures o f G1 and G3. Conclusion RV is a major cause of infantile viral diarrhea in daqing region, and it has smaller effects on adult diarrhea. Popular RV is given priority to with G1 and G3 in daqing region from 2009 to 2011. In addition,admixtures of Gl and G3 were found,which indicated some restructuring and variation happened between RV serotype.
出处
《医学研究杂志》
2014年第5期131-134,共4页
Journal of Medical Research
