摘要
背景:各种原因导致的较大骨缺损一直是临床修复的难题,建立标准的临界骨缺损动物模型,对评价各种成骨材料及技术的成骨效果具有重要的意义。目的:建立兔颅顶骨临界骨缺损模型,确定颅顶骨有限的时间内临界骨缺损值。方法:选取10只新西兰大白兔,以颅中缝为界,随机在颅顶骨上分别制备4个直径为4,5,6,7 mm的圆形全层骨缺损,建立兔颅顶骨临界骨缺损模型。通过大体解剖观察,X射线影像观察,锥形束CT观察并测定缺损区新生骨的骨密度,组织学检查,以评价各缺损区骨愈合情况。结果与结论:建模后12周,4 mm组骨自愈能力显著,部分出现完整的骨桥连接。骨密度定量分析和骨小梁面积检测结果显示,4 mm组缺损区骨密度灰度值和相同放大倍数且同一视野下分析骨小梁面积值均明显高于其他3组(P<0.001)。5,6,7 mm组只在缺损区周缘出现少量成骨,缺损中央区主要被纤维结缔组织所充填。结果证实,实验成功建立了兔颅顶骨临界骨缺损模型,在缺损12周的观察期内,直径≥5 mm的骨缺损不能自行愈合,符合临界骨缺损的标准,可作为兔颅顶临界骨缺损参考值。
BACKGROUND：Large bone defect caused by various reasons has been a difficult problem in clinical practice. To establish a standard experimental animal model of critical bone defects has vital significance for evaluating the efficacy of bone osteogenesis using various materials and techniques.
OBJECTIVE：To establish the rabbit model of parietal critical bone defects and to determine the diameter of the critical defects of parietal bone in limited time.
METHODS：10 New Zealand white rabbits were selected. The skul seam was treated as the boundary. Four ful-thickness round defects of bone in the parietal bone were made, with diameters of 4, 5, 6 and 7 mm, so as to establish rabbit models of parietal critical bone defects. Gross anatomical observation, X-ray and cone beam CT were used to determine the bone density in the new bone defect area. The healing of bone defects was evaluated by histological examination. RESULTS AND CONCLUSION：After 12 weeks, the 4 mm group showed high bone healing capacity significantly, and part of the bone bridge had been connected completely. Quantitative analysis of bone mineral density revealed that gray value at defect site and trabecular bone area at the same magnification and the same vision in the 4 mm group were significantly higher than the other three groups （P〈0.001）. Only a smal amount of new bone in the periphery of bone defects appeared in the 5, 6 and 7 mm groups. The center of defect site was mainly fil ed by fibrous connective tissue. The results confirmed that this study successful y established rabbit models of parietal critical bone defects. During the 12 weeks of observation, bone defects with a diameter of ≥ 5 mm could not be self-healed, which was conformed to the criteria of critical defects of bone, and could be used as a＆amp;nbsp;reference value for critical parietal bone defects of a rabbit.
出处
《中国组织工程研究》
CAS
CSCD
2014年第18期2789-2794,共6页
Chinese Journal of Tissue Engineering Research
基金
甘肃省科技计划项目(1208RJZA209)~~
