摘要
目的 探讨复方丹参注射液对感染性休克大鼠腑损伤时一氧化氮（NO）、B-细胞淋巴瘤-2 （bcl-2）蛋白及S100水平的影响.方法 选取2周龄健康清洁级Wistar大鼠40只,随机分为4个组,每组10只.感染性休克1组及感染性休克2组：通过尾静脉给予脂多糖（5 mg/kg）复制感染性休克模型;治疗组：造模成功后通过腹腔注射给予复方丹参注射液（5 mg/kg）;正常对照组：在其他组给予药物时给予9 g/L盐水替代.除感染性休克2组于造模成功12 h取材外,其余3组均于造模后6h取材.断头法取脑组织及摘眼球法取静脉血,用硝酸还原法检测4组大鼠脑组织中NO水平,免疫组织化学法检测4组大鼠海马组织bcl-2蛋白的表达,酶联免疫吸附法检测4组大鼠静脉血S100水平.结果 与正常对照组相比,感染性休克1组及治疗组NO、bcl-2及S100水平均明显升高,差异均有统计学意义（F=25.64、3 027.17、632.04,P均＜0.05）;治疗组NO及S100水平均较感染性休克1组明显降低,差异有统计学意义（F=25.64、632.04,P均＜0.05）,治疗组bcl-2表达水平较感染性休克1组明显增高,差异有统计学意义（F=3 027.17,P＜0.05）.感染性休克2组NO及S100水平较感染性休克1组均升高,差异均有统计学意义（t=-12.34、-4.89,P均＜0.05）,感染性休克2组bcl-2表达水平较感染性休克1组降低,差异有统计学意义（t=6.88,P＜0.05）.NO与bcl-2呈显著负相关（r=-0.928,P＜0.05）,NO与S100呈显著正相关（r=0.877,P＜0.05）.结论 感染性休克时,NO参与脑损伤的过程,可能与其下调bcl-2的表达,上调S100的表达有关;复方丹参注射液可能通过抑制NO生成,上调bcl-2表达,下调S100表达,对脑组织起保护作用.
Objective To explore the inlluence of compound Danshen injection on the expressions of nitric oxide （NO）,B-cell lymphoma-2 （bcl-2） protein and S100 in the septic shock rats with brain injury.Methods Forty 2-week-old healthy Wistar rats were randomly divided into 4 groups,10 cases in every group.Septic shock group 1 （6 h） and septic shock group 2 （12 h）：the rats were injected with lipopolysaecharide （5 mg/kg） via the tail vein to make the septic shock models;and the treatment group：rats were intraperitoneally injected with compound Danshen injection （5 mg/kg） immediately when shock signs appeared; Normal control group：the rats were given the same amount of 9 g/L saline while the other groups received drugs; The septic shock group 2 were drawn 12 h after the successful mode-ling,while the remaining 3 groups were havested at 6 h after the success of the modeling.The content of NO in the brain tissue was detected with nitrate reduction way,and the expression of bcl-2 in hippocampus was examined with immunohistochemistry techique,meanwhile the concentration of serum S100 was measured by enzyme linked immunosorbent assay.Results Compared with the normal control group,the contents of NO,bcl-2 and S100 in the septic shock 1 group increased significantly （F =25.64,3 027.17,632.04,all P 〈 0.05） ; however in treatment group,the contents of NO and S100 were less than those in the septic shock groups,while the level of bcl-2 was more than that in the septic shock groups,and there were significant diferences （F =25.64,632.04,3 027.17,all P 〈 0.05） ; compared with the septic shock group 1,the contents of NO and S100 in the septic shock group 2 increased drastically,but the level of bcl-2 in the septic shock 2 group was decreased,and there were significant differences （t =-12.34,-4.89,6.88,all P 〈 0.05）.NO and bcl-2 had significant negative correlation （r =-0.928,P 〈 0.05） ; NO had significant positive correlation with S100 （r =0.877,P 〈 0.05）.Conclusions NO participates in brain injury of septic shock rats,through inhibiting bcl-2 expression and up-regulating S100 expression.Compound Danshen injection has a protective effect on the brain tissues of rats during septic shock,through reducing the contents of NO as well as S100 and elevating the expression of bcl-2.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2014年第10期758-760,共3页
Chinese Journal of Applied Clinical Pediatrics
基金
江西省教育厅课题（GJJ 12096）
