摘要
目的:观察黄精对长期超负荷游泳模型大鼠的滋阴作用。方法:除正常组外,造模大鼠采用长期超负荷游泳建立阴虚内热模型,大鼠游泳8周,每周5 d,每天1次,日游泳时间由10 min逐日增加至第7周末180 min,水深60 cm,水温30℃。模型成功后,大鼠灌胃给予黄精水提物不同剂量(2.5,10 g·kg-1)连续8周。末次给药后眼眶取血,测大鼠血清免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、免疫球蛋白M(IgM)及白细胞介素2(IL-2)、白细胞介素(IL-6)含量,血浆环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)含量。给药期间同时观察大鼠一般行为学指标(体重、面温、痛阈值、抓力)。结果:与模型对照组比较,黄精水提物2.5,10 g·kg-1给药8周,能明显增加阴虚模型大鼠体重和抓力,降低面温及痛阈敏感性,升高血清IgA,IgM,IL-6含量,还能升高血清IgG的含量,但无统计学差异;黄精水提物10 g·kg-1还能升高血清IL-2含量,降低血浆cAMP含量及cAMP/cGMP。结论:黄精对长期超负荷游泳致阴虚模型大鼠行为学指标(体重、抓力、痛阈、面温)、免疫功能(血清免疫球蛋白IgA,IgG,IgM)、环核苷酸系统(血浆cAMP,cAMP/cGMP)均有改善作用,从而改善五心烦热、形体消瘦、腰膝酸软、免疫功能低下、环核苷酸系统紊乱等阴虚证候。
Objective： To observe the effect of Polygonatum sibiricum on Yin deficiency model rats induced by long-term overload swimming.Method： Except for the normal group,all of the remaining rats performed the long-term overload swimming for eight weeks,with five days every week and once every day,to establish the Yin deficiency model.The daily swimming time increased from10 min to 180 min at the end of the 7thweek,with the water depth of 60 cm and the water temperature at 30 ℃.After the success of the modeling,the rats were orally administered with different doses of aqueous extracts from P.sibiricum（2.5,10 g·kg- 1） for eight weeks.After the final administration,their blood were collected from orbits to measure immunoglobulin A,G and M（ IgA,IgG,IgM）,interleukin 2 and 6（IL-2,IL-6） and cAMP,cGMP contents in plasma.General behavioral indicators（weight,facial temperature,pain threshold and holding power） of rats were observed during the drug administration.Result： Compared with the model control group,aqueous extracts from P.sibiricum was given for eight weeks to significantly increase the rat weight and holding power of Yin deficiency model rats,decrease the facial temperature and the sensitivity of pain threshold,and increase IgA,IgG,IgM and IL-6 content and IgG content in serum,but without statistical difference.Aqueous extracts from P.sibiricum（10 g·kg- 1） could also increase IL-2 content in serum,and decrease cAMP content and cAMP /cGMP ratio.Conclusion： P.sibiricum could improve the general behavioral indicators（weight,holding power,pain threshold and facial temperature）,immunologic functions（ IgA,IgG,IgM） and cyclic nucleotide（cAMP,cAMP /cGMP）,so as to ameliorate such Yin deficiency symptoms as dysphoria in chestpalms-soles,weight loss,soreness and weakness of waist and knees,immunologic dysfunction and cyclic nucleotide system disorders.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第10期1886-1891,共6页
China Journal of Chinese Materia Medica
基金
国家重点基础研究发展计划(973)项目(2007CB512603)
关键词
黄精
超负荷游泳
大鼠
阴虚
模型
Polygonatum sibiricum
overload swimming
rat
Yin deficiency
model
