摘要
采用液相色谱-大气压化学电离质谱(HPLC-APCI-MS)方法,分析了食用油脂和地沟油的甘油三酯(TAG)组成。采用C18色谱柱,乙腈-丙酮为流动相,梯度洗脱,APCI正离子模式检测。应用本方法检测了15个地沟油和11个食用油样品,结果表明,地沟油中存在三亚油酸甘油酯(LLL)、二亚油酸单油酸甘油酯(LOL)、二亚油酸单亚麻酸甘油酯(LLnL),二油酸单亚油酸甘油酯(OOL)等甘油三酯的5种亚油酰基氧化产物。采用偏最小二乘法判别分析(PLS-DA)化学计量学方法,分析建立了地沟油和正常食用油的判别模型,实现了正常食用油和地沟油区分,模型正确判别率96.2%。在判别模型中,含有亚油酸环氧化物的TAG分子对判别贡献率较大,该类化合物可作为地沟油区别于正常油脂的标志成分。
Triacylglycerols （ TAGs） in edible oils （ soybean, peanut, sesame） and hogwash oil were analyzed by HPLC-MS with atmospheric pressure chemical ionization （ APCI ） . The gradient elution chromatographic separation was performed on a C18 column using mobile phase of acetonitrile-acetone, and TAGs were monitored by APCI-MS in positive mode. 15 hogwash oils and 11 normal edible oils were analysed with the method. Five linoleoyl epoxides of trilinolein （ LLL） , dilinoleoyl-oleoyl glycerol （ LOL） , dilinoleoyl-linolenoyl glycerol （ LLnL） , dioleoyl-linoleoyl glycerol （ OOL） in hogwash oil were detected. The discriminantion model established with partial least square discriminate analysis （ PLS-DA） , could distinguish the normal edible oil and the hogwash oil with an accuracy of 96 . 2%. The linoleoyl epoxides of TAGs made the major distinguishing contribution for the model, and were proved as the mark components in hogwash oil different from the normal edible oil.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2014年第5期741-746,共6页
Chinese Journal of Analytical Chemistry
基金
辽宁省科技计划项目(No.2010415015)
沈阳市科技计划项目(No.F12-162-9-00)资助
