摘要
目的:探讨针刺对抑郁症外周和中枢炎性细胞因子的调节作用。方法:采用慢性不可预知的应激方法建立抑郁症大鼠模型。将SD大鼠随机分为正常组、模型组、针刺组和氟西汀组。采用酶联免疫吸附法(ELISA)检测炎性细胞因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的含量。结果:与正常组比较,模型组大鼠血清、海马、额叶皮层IL-1β含量均显著升高(P<0.01),血清、海马、额叶皮层IL-6含量均显著升高(P<0.01),血清、海马、额叶皮层TNF-α含量均显著升高(P<0.01)。针刺治疗可以显著降低模型大鼠血清、海马和额叶皮层IL-1β、IL-6和TNF-α含量(P<0.01)。结论:针刺可以抑制炎性细胞因子,缓解抑郁症外周和中枢炎症反应,这可能是针刺发挥抗抑郁作用的机制之一。
Objective ：To observe the effect of acupuncture on inflammatory cytokines response in serum and brain regions of depression. Methods：The chronic unpredictable stress （CUS） rat model with depression was employed in this study. The SD rats were randomly divided into four groups： the normal control, CUS, acupuncture and fluoxetine. The contents of pro - inflammatory cytokines interleukin - 1β （ IL - 1β）, IL - 6 and tumor necrosis factor-α（TNF- α） were detected by ELISA. Results：Compared with control group, the contents of IL - 1β in serum, hippocampus and frontal cortex in model group were significantly increased （P 〈 0. 01）; the contents of IL- 6 in serum, hippocampus and frontal cortex were significantly increased （P 〈 0. 01 ） ; the contents of TNF - αin serum, hippocampus and frontal cortex were significantly increased （P 〈 0. 01 ）. Acupuncture significantly decreased the contents of IL - 1β, IL - 6 and TNF -α in the serum, hippocampus and frontal cortex of the model group （P 〈 0.01 ）. Conclusion：Acupuncture treatment could significantly reduce the inflammatory cytokines in serum and brain, and alleviate inflammatory response, which might mediate the antidepression by acupuncture.
出处
《针灸临床杂志》
2014年第5期57-60,共4页
Journal of Clinical Acupuncture and Moxibustion
基金
国家自然科学基金项目
编号:81102638
81173334
北京中医药大学自主选题项目
编号:2011JYBZZ-JS067
关键词
针刺
慢性应激
抑郁症
细胞因子
Acupuncture
Chronic stress
Depression
Cytokines
