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6株超级细菌NDM-1基因环境研究 被引量：7

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摘要 目的:研究6株超级细菌NDM-1基因环境,探讨耐药基因水平转移机制。方法:收集耐碳青霉烯的G-杆菌400株,利用PCR方法检测NDM-1基因,并测序验证。根据目前报道的NDM-1基因环境常见类型设计系列引物,步移法特异性扩增NDM-1基因上下游序列,并对扩增产物测序和序列分析。步移法检测阴性的菌株进行全基因组HindⅢ酶切,将NDM-1基因及基因环境克隆到pET28a质粒后再测序。结果:400株对碳青霉烯类耐药的G-杆菌中有6株NDM-1阳性。步移法检出其中5株菌的基因环境,包括不动杆菌基因种13TU、产酸克雷伯菌、不动杆菌基因种3、肺炎克雷伯菌和阴沟肠杆菌。这5株菌的基因环境与文献报道基本一致。产气肠杆菌的NDM-1基因环境经DNA克隆测序获得,从上游至下游为:部分缺失的转座酶Tn3、IS26及ISAba125,blaNDM-1,ble,部分缺失的异构酶trpF和SSen4元件。结论:NDM-1基因上下游常包含转座子或插入序列等元件,为其水平转移奠定基础。
作者 程灿灿 芮勇宇
机构地区 南方医科大学南方医院检验科
出处 《实用医学杂志》 CAS 北大核心 2014年第11期1820-1823,共4页 The Journal of Practical Medicine
基金 广东省自然科学基金项目(编号:S2012010009153)
关键词 超级细菌 NDM-1 基因环境
分类号 R440 [医药卫生—诊断学]
  • 相关文献

参考文献16

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二级参考文献33

  • 1Kumarasamy KK,Toleman MA,Walsh TR,et al. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK:a molecular,biological, and epidemiological study[J]. Lancet Infect Dis,2010,10(9) :597-602.
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共引文献28

同被引文献109

  • 1莫岚.青霉素结合蛋白与细菌对β-内酰胺类抗生素耐药机制的研究进展[J].国外医药（抗生素分册）,1996,17(3):187-191. 被引量：8
  • 2徐辉,徐玉兰,马红梅.碳青霉烯类抗生素的研究进展[J].沈阳药科大学学报,2007,24(6):385-388. 被引量：11
  • 3Yong D,Toleman MA,Giske CG, et al. Characterization of a new metallo-beta-lactamase gene, bla ( NDM-1 ), and a novel erythro- mycin esterase gene carried on a unique genetic structure in leb siella pneumoniae equence type 14 from India[J]. Antimicroh A- gents Chemother,2009,53(12) :5046 5054.
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  • 7Kumarasamy KK,Toleman MA,Walsh TR,et al. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK ~ a molecular, biological, and epidem iologicalstudy [ J ]. Lancet Infect Dis,2010,10(9) :597-602.
  • 8ChenY,Cui Y J, Pu F,et al. Draft genome sequence of an Aeineto- bacter genomic species 3 strain harboring a bla(NDM-1) gene[J]. J Baeteriol,2012,194 (1) :204-205.
  • 9Wu HS, Chen TL.Chen IC, et al. First identification of a patient colonized with Klebsiella pneumoniae carrying blaNDMq in Tai wan[J]. J Chin Med Assoc,2010,7(ll):596-598.
  • 10Nordmann P, Naas T, Poirel L. Global spread of Carbapenemase- producing Enierobateriaceae[J]. Emerg Infect Dis. 2011,17 (10 ) : 1791-1798.

引证文献7

二级引证文献33

实用医学杂志
2014年 第11期

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