摘要
目的为了更好的控制骨科手术切口感染以及更合理的控制临床用药,对医院骨科病房患者病原菌分布以及耐药性进行分析。方法对2006年12月-2013年2月骨科病房350例患者手术切口感染标本进行病原菌分离,采取K-B纸片扩散法敏感试验,采用的法国生物梅里埃公司的VITEK-60全自动细菌分析仪进行病原菌鉴定,采用K-B纸片扩散法进行耐药性分析,将所有数据应用SPSS11.0软件进行统计分析。结果 350例骨科手术切口感染患者标本主要来源于分泌物或脓液,共943份占89.91%,其次,血液标本37份占3.43%;共培养出病原菌1 050株,其中革兰阴性杆菌682株占64.95%,革兰阳性球菌196株占18.67%,真菌172株占16.38%;铜绿假单胞菌、不动杆菌属、大肠埃希菌对亚胺培南的耐药率较低,分别为69.94%、16.43%、8.33%,革兰阳性球菌对万古霉素、替考拉宁的耐药率均为0,真菌的耐药率普遍较低。结论骨科患者手术切口感染病原菌分布要随时检测,根据监测结果合理使用抗菌药物,以此减少新耐药株的形成,同时降低医院感染率。
OBJECTIVE To explore the distribution and drug resistance of pathogens isolated from the patients from wards of department of orthopedics so as to control the surgical incision infections and reasonable use of antibiotics .METHODS From Dec 2006 to Feb 2013 ,the pathogens were isolated from the specimens obtained from 350 patients with surgical incision infections in wards of orthopedics department , then the drug susceptibility testing was performed with the use of K-B disk diffusion method ,the isolated pathogens were identified by using VITEK-60 automatic bacteria analyzer of BioMérieux ,France ;the drug resistance was analyzed by means of K-B disk diffusion method ,and all the obtained data were statistically analyzed by using SPSS 11 .0 software .RESULTS The secretions and pus were the predominant specimen sources that were obtained from the 350 patients with surgical incision infections of the orthopedics department ,943 in total ,accounting for 89 .91% ,and 37 blood specimens were obtained , accounting for 3 .43% . A total of 1050 strains of pathogens have been isolated , including 682 （64 .95% ） strains of gram-negative bacilli ,196 （18 .67% ） strains of gram-positive cocci ,and 172 （16 .38% ） strains of fungi .The drug resistance rates of the Pseudomonas aeruginosa ,Acinetobacter ,and Esche-richia coli to imipenem were 69 .94% ,16 .43% ,and 8 .33% ,respectively ;the drug resistance rates of the gram-positive cocci to vancomycin and teicoplanin were 0 ; the drug resistance rates of the fungi were low . CONCLUSIONS It is necessary to detect the distribution of pathogens causing surgical incision infections in a timely manner and reasonably use antibiotics based on the result of surveillance so as to prevent the emergence of novel drug-resistant strains and reduce the incidence of nosocomial infections .
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2014年第12期2913-2915,共3页
Chinese Journal of Nosocomiology
基金
衢州市科技局研究基金资助项目(2013085)
关键词
切口感染
骨科手术
病原菌
耐药性
Incision infection
Orthopedic surgery
Pathogen
Drug resistance
