摘要
目的探讨结直肠癌组织中STAT3及其磷酸化STAT3（P-STAT3）和E钙黏蛋白（E—cadherin）表达的相关性及其与肿瘤浸润转移的关系。方法用免疫组织化学Elivision^TM plus法检测江西赣南医学院第一附属医院病理科存档的50例结直肠癌组织及相应癌旁组织中STAT3、P-STAT3及上皮细胞间质化（EMT）相关蛋白E—cadherin的表达，并分析其与结直肠癌临床病理特征的关系。结果50例结直肠癌组织中STAT3和p-STAT3及E—cadherin的阳性表达率分别为72％（36／50）、76％（38／50）和26％（13／50），相应癌旁组织中的表达则分别为24％（12／50）和26％（13／50）及68％（34／50），结直肠癌组织STAT3和p-STAT3的表达明显高于相应癌旁组织，而E—cadherin的表达则明显低于癌旁组织（均P〈0．05）。STAT3、P-STAT3及E．cadherin表达与肿瘤浸润深度、分化程度、肿瘤大小、淋巴结转移及TNM分期有关（均P〈0．05）。STAT3和P—STAT3蛋白在结直肠癌中的表达与E-cadherin呈显著负相关（均P〈0．05）。结论STAT3和p-STAT3可能通过对E—cadherin的抑制作用进而引起EMT现象，从而导致结直肠肿瘤的发生和进展。
Objective To investigate the correlation of expressions of STA33 and p-STAT3 with epithelial mesenchymal transition （EMT）-associated protein E-cadherin in colorectal cancer, and to examine the association of above expressions with tumor invasion and metastasis of colorectal cancer. Methods Immunohistochemistry assay ElivisionTM plus was used to detect the expressions of STAT3, p- STAT3 and E-cadherin protein in colorectal cancer tissue samples of 50 cases and their corresponding adjacent non-tumor tissues. Association of these protein expressions with tumor invasion and metastasis was analyzed with X2 test. Correlation of STAT3 and p-STAT3 with E-cadherin was analyzed with Spearman method. Results Positive expression rates of STAT3, p-STAT3 and E-cadherin protein in colorectal cancer tissues were 72%（36/50）, 76%（38/50） and 26%（13/50）, which were significantly higher compared to adjacent normal intestinal mucosa tissues [24%（12/50）, 26%（13/50） and 68% （34/50）, all P〈0.05]. STAT3, p-STAT3 and E-cadherin expressions were associated with tumor differentiation, tumor invasion depth, tumor size, lymph node metastasis, TNM staging （all P〈0.05）. In colorectal cancer tissues, STAT3 protein expression was positively correlated with p-STArI3 expression. STAT3 and p-STAT3 expressions in colorectal cancer tissues were negatively correlated with E-cadherin expression （P〈0.05）. Conclusion STAT3 and p-STAT3 may be involved in tumor EMT through inhibition of E-cadherin expression, leading to the development of colorectal cancer.
出处
《中华胃肠外科杂志》
CAS
CSCD
2014年第6期594-597,共4页
Chinese Journal of Gastrointestinal Surgery
基金
国家自然科学基金（81000908）
江西省教育厅一般项目（GJJ14687）
赣南医学院基础研究项目（ZD201314）
