摘要
采用室内暴露实验方法,研究体积分数分别为0,10%,30%,50%和70%的链霉素生产废水15d内对斑马鱼肌肉组织超氧化物歧化酶(SOD)和丙二醛(MDA)含量的影响。结果显示:斑马鱼肌肉SOD活性在第3天受到显著诱导,体积分数50%实验组SOD活性达到最大值30.56U/mg。体积分数70%实验组SOD活性随着暴露时间的延长逐渐降低。体积分数30%和50%实验组MDA活性在第3天受到显著诱导,在第6天大幅度下降,降幅分别为43.04%和47.50%,第12天和第15天,MDA活性都高于对照组。暴露期间体积分数10%和70%实验组的MDA活性高于对照组。实验表明,鱼体肌肉组织SOD活性和MDA含量在暴露过程中变化明显,该链霉素生产废水已经对斑马鱼产生了氧化损伤效应。
An exposure experiment is carried out to study the effect of 0, 10%, 30%, 50% and 70% （V/V） streptomycin wastewater from a workshop on superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in muscles of zebra fish for 15 days. The results show that SOD activity is significantly induced on the third day, and the highest expression of SOD reaches 30.56 U/mgprot in 50% group. SOD activity in 70% group is gradually reduced and in a dose-time manner. MDA activity of 30% and 50% group are significantly induced on the third day and drop dramatically on the sixth day, namely 43.04% and 47.50%, respectively, while MDA activity is higher than the control group on the twelfth day and fifteenth day. During the exposure, MDA activity of 10% and 70% group are higher than the control group. It is suggested that SOD activity and MDA content in the exposure process chang apparently, and the streptomycin wastewater causes oxidative damage on zebra fish.
出处
《河北科技大学学报》
CAS
2014年第3期303-308,共6页
Journal of Hebei University of Science and Technology
基金
国家环保部公益课题(201109004)
河北省自然科学基金(B201408068)
河北省教育厅重点课题(ZH2011119)
河北省环保厅公益课题
河北省重点学科建设基金
河北科技大学五大平台开放基金
