摘要
目的探讨急进高原大鼠肺泡液体清除率(ALC)的变化,及肺泡Ⅱ型细胞(ATⅡ)肺水转运功能与肺损伤的关系。方法用I125-白蛋白为示踪剂进行肺灌注检测ALC。将60只健康Wistar大鼠随机分4组,每组15只。其中对照组(T0组)在平原进行实验检测,另外3组分别于急进高原24 h(T1组)、72 h(T2组)、168 h(T3组)测量ALC。解剖取出肺组织,经处理后用光镜观察细胞形态并作电镜超微结构检查。结果 T1组、T2组和T3组ALC分别为22.60±3.41%、25.28±6.30%、45.52±10.05%。T1组、T2组与T3组比较有统计学意义(均P<0.05),T3组与T0组比较无显著性差异(P>0.05)。光镜下,各组大鼠肺组织呈明显炎症反应,肺泡壁毛细血管充血水肿,凋亡细胞在肺组织中呈单个散在分布,坏死组织呈均匀红染无结构的颗粒状,核染色质消失。电镜下ATⅡ核固缩,大多数细胞微绒毛减少或消失,部分ATⅡ凋亡,尤其T1组、T2组病变明显。结论急进高原大鼠出现肺泡Ⅱ型细胞凋亡,肺泡上皮细胞的数量明显减少,肺水转运功能障碍,肺清除率下降,大量的水肿液积聚在肺泡腔内,可引发高原肺水肿。
Objective To investigate alveolar liquid clearance （ALC） changes of the rat by reaching the accelerated high altitude, explore the relationship between lung water transport function of the alveolar type Ⅱ （AT Ⅱ ）ceils and lung injury. Methods I^125-the albumin as tracer detection of lung perfusion ALC. The 60 healthy Wistar rats were randomly divided into four groups with 15 case each group. Among the control group （TO group） carried out experiments to detect the plains, the other three groups were stationed high altitude and were respectively measured one hour of ALC at 24 h （T1 group） , 72 h （T2 group） , 168 h （T3 group）. Anatomy and remove lung tissue, to observe the uhrastructure after processing by electron microscope. Results When the Wistar rats reach high altitude , the alveolar liquid clearance at 24 hours was 22.60 ± 3.41% , 72 hours was 25.28 ± 6.30%, 168 hours was 45.52 ± 10.05%. There were significant differences when 24 hours, 72 hours groups compared with normal group. There were no significant difference when 168 hours group compared with normal group. There were blue-black nuclei and pale red cytoplasm by Light microscope. Necrotic tissue was stained red uniform unstructured and granular chromatin disappear. It was appared lung tissue inflammation and alveolar capillary wall edema. Apoptotic cells were scattered in a single lung tissue. It showed nucleus pycnosis of the AT Ⅱ cell under electron microscope, microvilli decreased or disappeared. It appeared apoptosis in the part of the the AT Ⅱ cell. Conclusions It showed apoptosis of the AT Ⅱ cell of the rat in acute high altitude, and significantly reduced the number of alveolar epithelial cells. It appeared lung water transport dysfunction and alveolar liquid clearance reducing. A lot of edema fluid accumulation in the alveolar cavity may lead to high altitude pulmonary edema.
出处
《中华肺部疾病杂志（电子版）》
CAS
2014年第3期34-36,共3页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
青海大学中青年基金项目(2012-QYY-2)
国家自然科学基金项目(81160012)
国家卫生部卫生行业专项(201002012)
