摘要
为了优化和验证葛根芩连注射剂中葛根素、黄芩苷、盐酸小檗碱3种成分的高效液相色谱（HPLC）测定方法，选用Kromsil ODS（250mm×4．6mm，5μm）色谱柱，流动相分别为甲醇-水（30：70，V／V）、甲醇-水-磷酸（40：60：0．2，V／V）、乙腈-水-磷酸二氢钾（32：68：0．3，V／V），检测波长分别为251、280、348nm，柱温25℃，流速1．0mL／min。结果显示，葛根素在0．7～4．2／zg／mL有良好的线性关系，平均回收率为98．8％；黄芩苷在4．3～25．8μg／mL有良好的线性关系，平均回收率为95．3％；盐酸小檗碱在4．0～24．0μg／mL有良好的线性关系，平均回收率为96．4％。葛根芩连注射剂中葛根素、黄芩苷、盐酸小檗碱含量分别为2．086、2．573、6．836mg／mL，且批内变异系数均小于2％，批间变异系数均小于3％，符合检测要求。表明优化后的HPLC法准确、简便、快速，可用于葛根芩连注射剂的质量控制和评价。
In order to establish a simple HPLC method for determining the content of three virtual components in Gegenqinlian injection,Kromsil ODS column（250 min×4.6 mm,5 μm） was used. The HPLC system for puerarin,baicalin,berberine used the mobile phase composed of methanolwater（30 ： 70,V/V）,methanol-water-phosphoric acid（40 ： 60 ： 0. 2,V/V） and acetonitrile-water-potassium dihydrogen phosphate（32 ： 68 ： 0.3,V/V）,respectively,with a flow rate of 1.0 mL/min and the column temperature was maintained at 25 ℃. The detection wavelength was set at 251 nm, 280 nm and 348 nm, respectively. The results showed that the puerarin calibration curve was linear in the range of 0. 7-4.2 μg/mL with the average recoveries was 98.8%. The calibration curve of baicalin was linear in the range of 4.3- 25.8 μg/mL with the average recoveries was 95.3%. The calibration curve of berberine was linear in the range of 4.0-24.0 μg/mL with the average recoveries is 96.4%. The content of puerarin, baicalin and berberine was controlled as qualified more than 2. 086 mg/mL, 2. 573 mg/mL and 6. 836 mg/mL, with CV for within-run assays was less than 2 % and CV for between-run assays was 3 %, respectively. This method was accurate,convenient and quick. It can be used for Gegenqinlian injection quality control.
出处
《河南农业科学》
CSCD
北大核心
2014年第6期152-156,共5页
Journal of Henan Agricultural Sciences
基金
四川省教育厅重点项目(11ZA085)
