摘要
研究了温度和光生物反应器的光径对微绿球藻(Nannochloropsis oculata Hibberd)生长及细胞内几种营养成分含量的影响。结果表明,温度对微绿球藻的生长有显著影响(P<0.05),对几种营养成分的含量有极显著影响(P<0.01),在28℃的培养条件下,细胞生长速度最快,此时蛋白质含量最高,为5.66%,多糖含量最低,仅为1.47%,但最大生物产量却是在30℃的条件下获得,总脂与叶绿素a的含量随温度的升高而降低,类胡萝卜素含量随温度升高而增大。光生物反应器的光径对微绿球藻的生长和总脂、可溶性蛋白、色素的含量有极显著影响(P<0.01),对多糖含量有显著影响(P<0.05),比生长速率、最高细胞密度和单位体积产量均随光径的增大而减小,单位面积产量随光径的增大而增大;总脂、可溶性蛋白和多糖的含量随光径的增大而降低,叶绿素和类胡萝卜素含量随光径增大而增大,类胡萝卜素含量的增大趋势比叶绿素更明显。
The effect of temperature and optical path of flat plate glass reactors on growth rate and organics con-tents of Nannochloropsis sp. was investigated, using a range of optical paths from 5 to 20 cm. The results showed that the temperature had a significant effect on the growth of Nannochloropsis （P〈0.05）, and a very significant ef-fect on the contents of mutrients （P〈0.01）. The cell growth had a highest speed at 28℃, when the content of soluble protein reached the maximum and that of polysaccharides reached the minimum. The biomass yield reached the maximum at 30℃. The contents of lipid and chlorophyll decreased with the increase of temperature, while the con-tent of carotenoid increased with the increase of temperature. The optical path of photobioreactors had a very sig-nificant effect on the growth of Nannochloropsis （P〈0.01）. Specific growth rate, maximal cell density （107cell/mL） and yield per unit volume （mg /（L·d） ） decreased with increase in optical path. Yield per unit area （g /（m2·d） ） in-creased with increased optical path. The contents of lipid, soluble protein, and polysaccharides decreased with in-creased optical path, but the contents of chlorophyll and carotenoid increased with increased optical path. The in-crease trend of carotenoids was more obvious than that of chlorophyll. In summary, the optimal optical path deter-mines the optimal culture density, as well as yield per unit volume and the productivity of organics. Under our con-ditions the optimal optical path for culturing Nannochloropsis to accumulate lipid, protein and polysaccharide in plate reactors was 5 cm, the optimal optical path for accumulating pigments was 20 cm.
出处
《海洋科学》
CAS
CSCD
北大核心
2014年第4期50-54,共5页
Marine Sciences
