摘要
运用响应曲面法优化铜藻多糖(tong zao polysaccharide,TZP)微波辅助提取的工艺条件,利用分级醇沉法得到TZP30%、TZP60%和TZP80%3个多糖组分。并对其DPPH自由基、ABTS自由基、OH自由基和O2-自由基的清除能力及其还原力进行了研究。结果表明:铜藻多糖微波辅助提取的最佳条件为料液比1:65,提取温度72℃,提取时间39min,多糖提取率为12.02%(n=3);在试验浓度范围内,TZP30%组分对羟基自由基的清除能力最高达98.07%;TZP60%组分对DPPH自由基清除能力最高达85.01%;TZP80%组分对ABTS自由基清除能力较强,还原力较大。铜藻多糖具有较高的抗氧化活性,具有作为天然抗氧化剂的应用前景。
The microwave-assisted extraction condition of polysaccharides from Sargassum horneri （TZP） was optimized by response surface methodology（RSM）. Three kinds of crude polysaccharides with different components （TZP30% , TZP60% , TZP80% ） were obtained through stepwise ethanol precipitation. The purified polysaccharides were evaluated on their antioxidant activities in vitro systems including DPPH assay, superoxide radical assay, hydroxyl assay, ABTS assay and reducing capacity assay. The optimal extraction conditions obtained by RSM were as follows： solidliquid ratio 1：65, extraction temperature 72~C, extraction time 39min. Under the optimal extraction conditions, the relative extraction rate of SHP could reach 12.02%. （n = 3 ）. The Antioxidant activities of TZP were determined by spectrophotometry. The results showed that TZP30% showed high scavenging effect on OH· and O2^ - , TZP60% showed high scavenging effect on DPPH., and TZP80% showed high scavenging effect on ABTS and high reducing power. So, polysaccharides from Sargassum horneri were promising for future use in natural antioxidant industry.
出处
《核农学报》
CAS
CSCD
北大核心
2014年第6期1062-1069,共8页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(31301560)
浙江省重大科技项目(2011C12040)
关键词
铜藻多糖
微波辅助提取
响应曲面法
抗氧化活性
Polysaccharides from Sargassum horneri
Microwave-assisted
Response surface methodology
Antioxidantactivities
