摘要
参考新城疫病毒 ( NDV)长春株的 F基因序列设计了 1对特异性引物 ,应用 RT-PCR对 NDV青岛株(野毒 )的 F基因进行了扩增 ,扩增产物克隆后测序。扩出的 F基因核苷酸长度为 792 bp,编码 2 6 1个氨基酸 ,包括完整的 F2片段和部分 F1片段。裂解位点区 ( 1 1 2～ 1 1 7aa)氨基酸序列为 Arg-Arg-Gln-Arg-Arg-Phe,与国外发表的强毒株序列相符 ,证明青岛株为强毒株。根据该基因推导的氨基酸序列 ,与国内外发表的 NDVF蛋白氨基酸序列相比 ,同源性为 88.1 %～ 94 .3%。
A pair of special oligonucleotide primers were generated by F gene sequence of NDV strain Changchun and F gene of NDV strain Qingdao was amplified by RT-PCR.The F gene was sequenced after being directly inserted in recombinant plasmid pKSC.The nucleotide sequence of this F gene was 798 bp long encoding 261 amino acids.It contains entire F2 fragment and a part of F1 fragment of F gene.The amino acid sequence of cleavage site region(residues 112-117 aa)is Arg-Arg-Gln-Arg-Arg-Phe matching to that of all virulent NDV strain(Arg-Arg/Lys-Gln-Arg/Lys-Arg-Phe).This proved that NDV strain Qingdao must be a virulent strain.Compared with the published NDV strain,the homology of the deduced amino acid sequence is between 88.1%-94.3%。
出处
《中国兽医学报》
CAS
CSCD
北大核心
2001年第2期113-116,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金重大项目! (398932 90 )
