摘要
对灵芝原生质体进行了紫外诱变处理 ,经过粗筛和精筛后 ,从中选出多糖含量和产量明显高于原始菌株的两株诱变株 430 2 0 #和 430 2 6#。经过 1 0代PDA斜面继代培养及其摇瓶试验和连续 3次 3t罐的中试试验 ,表明所得诱变株为比原始菌株更优秀的稳定高产、高多糖含量的灵芝生产菌株。本研究为选育适合发酵的灵芝生产菌种提供了一种快速。
UV induced mutagenesis of Ganoderma 's protoplast was carried out.By crude screen and careful screen,we obtained two mutants,43020# and 43026#,whose dry weight and polysaccharides contents are higher than original strain.Results of continued cultivation for 10 generation on PDA slants and successive tests of shaking bottles,and successive 3 time pilot scale tests at 3 tons,showed that these two mutants are excellent strains with steady properties,high production and high content of polysaccharides.This study has provided a rapid and effective method for breeding of Ganoderma varieties which is suitable to industrial fermentation.
出处
《微生物学报》
CAS
CSCD
北大核心
2001年第2期229-233,共5页
Acta Microbiologica Sinica
基金
上海中祥生物制品有限公司资助
