摘要
目的 :建立获得性多药耐药 (MDR)表达小鼠腹水模型 ,探讨检测S180细胞和外周血淋巴细胞MDR1表达水平及凋亡指标的意义。方法 :给予S180细胞荷瘤小鼠反复定量联合化疗刺激 ,用流式细胞术动态检测S180细胞和同期外周血淋巴细胞MDR1表达水平及凋亡指标结果 :( 1)实验组小鼠S180自化疗后 1周MDR1表达逐渐增高 ,第 5周后显著增加 (P <0 .0 5) ;而细胞凋亡率、细胞杀伤率及Fas因子表达率则随化疗时间的延长而逐渐下降 ,且MDR1表达显著增加时 ,S180细胞凋亡率等则显著降低 (P <0 .0 5) ;( 2 )实验组外周血淋巴细胞MDR1、细胞凋亡水平与S180细胞显著相关。结论 :小鼠S180细胞多药耐药模型有助于获得在体研究MDR1表达的经验 。
Objective:To establish the mouse model of MDR1 expression in ascites with S180 cell and study the method to indicate the MDR1 expression(P 170 ) and apoptotic parameters in ascites with S180 cells and the peripheral lymphocyte.Methods:The mice with S180 cell in ascites were stimulated with repeated-combined-chemotherapy(RCC),the contents of MDR1 expression(P 170 ) and apoptotic parameters were repeatedly assayed by flow cytometry.The above parameters of peripheral lymphocyte(PL)were assayed at the same time.Results: (1)The cellular MDR1 expression(p 170 content)of mice in study group were gradually improved after one week's RCC,and there was statistically difference after five weeks from that in control group;By the mean time,the cellular apoptotic rate,cellular-killed rate and Fas factor expression were gradually decreased along with the progress of RCC;(2)In study group the MDR1 expression and apoptotic rate of PL of mice were statistically correlated with those of S180 cells. Conclusions:The mouse model with obtained(secondary)cellular MDR1 expression of S180 cell line is helpful to study the obtained multidrug resistance to chemotherapy in clinic,and the MDR1 expression level of S180 cell could be correlated to those of the peripheral lymphocyte during the later period in chemotherapy.
出处
《现代妇产科进展》
CSCD
2001年第2期95-98,共4页
Progress in Obstetrics and Gynecology
关键词
疾病模型
药物疗法
多药耐药基因
细胞凋亡
肿瘤
Disease models,animal
Drug therapy
Multidrug resistant gene
Cellular apoptosis
