摘要
目的 研究羟基喜树碱 (HCPT )对家兔环孢素A(CsA)药物动力学及对红细胞膜流动性 (LFU)的影响。方法 用TDX快速测定CsA全血药物浓度 ,用DPH荧光探针法测定红细胞膜流动性。结果 HCPT可使CsA的a ,V(c) ,K12 及CL(s)降低 ,T1/ 2 (a) 增加 (P <0 0 5 )。联合给药组的CsA血药浓度在给药后的 0 2 5 ,0 5和 1h高于单独给药组 ,且在 1h时差异有显著性 (P <0 0 5 )。联合给药组的LFU值在给药后的 0 5 ,1 0 ,1 5h时高于单独给药组 (P <0 0 5 ) ,与对照组差异无显著性。单独给药组的LFU值在给药后的 0 5 ,1 0 ,1 5h时低于对照组。结论 HCPT与CsA合用可提高CsA的血药浓度 。
AIM To investigate the effect of hydroxyl camptothecinum(HCPT) on pharmocokinetics of cyclosprine A(CsA) and the membrane lipid fluidity (LFU)of red cell in rabbits. METHODS The whole blood concentration of CsA in rabbits was monitored using method of FPIA. The LFU of red cell was measured using method of DPH fluorescence polarization technique. RESULTS The pharmocokinetic parameters of CsA in group CsA+HCPT such as a, V (c), K 12 and CL (s) were significantly lower and T 1/2(a) was significantly higher ( P <0 05) than in group CsA. The concentration of CsA of group CsA+HCPT was higher after injection 0 25, 0 5 and 1 0 h, but have significantly high at 1 0 h point ( P <0 05). After injection 0 5,1 0, 1 5 h the LFU of red cell in group CsA+HCPT was higher than in group CsA ( P <0 05). The LFU in group CsA was lower that in control at 0 5,1 0 and 1 5 h point ( P <0 05). CONCLUSION HCPT can enhance the whole blood concentration of CsA in rabbits. CsA administrated with HCPT is feasible.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2001年第1期94-96,共3页
Chinese Pharmacological Bulletin
