摘要
目的 :探讨内毒素血症在大鼠肝硬化发生发展过程中的作用。方法 :采用饮水中加入硫代乙酰胺(TAA) ,另一组同时小剂量注射脂多糖 (LPS) ,以观察其对肝硬化形成的影响。结果 :TAA可使动物血浆内毒素水平与肝脏胶原蛋白含量明显增高 ,且二者存在直线相关 ;血浆和肝组织中肿瘤坏死因子α(TNFα)、内皮素 - 1(ET - 1)、一氧化氮 (NO)、丙二醛 (MDA)含量均增加 ,而且血浆中各成分含量均与内毒素血症呈正相关 ;在TAA +LPS组动物唯有TNFα浓度和胶原蛋白含量明显高于TAA组。结论 :在肝硬化形成过程中内毒素可能主要通过激活枯否细胞分泌TNFα发挥其作用 ,但ET - 1、NO和自由基也参与其中。
AIM: To study the roles of endotoxemia in the occurence and development of cirrhosis. METHODS: The cirrhosis of rats were made by oral intake of thioacetamide (TAA-treated rats). A separate group of rats were subjected to a injection of small dosage of lipopolisaccharride (LPS) in the period of thiocetamide treatment in order to observe the possible effect of LPS on the forming of cirrhosis. RESULTS:Both plasma endotoxin levels and hepatic collagen contents were increased in TAA-treated rats,and there was a linear correlation between them ;Plasma and hepatic tumor necrosis factor (TNFα)、endothelin-1(ET-1)、nitric oxide(NO)、and MDA levels were all increased,and plasma endotoxin was correlated with all of their plasma contents;Only TNFα levels and hepatic collagen contents of TAA+LPS group were increased significantly compared with TAA group. CONCLUSION: These results suggest that endotoxemia (ETM) could play an important role in the development of cirrhosis mainly through activating Kupffer cells, which secrete TNFα, ET-1, NO and free radicals could be involved in the process.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第4期353-355,T001,共4页
Chinese Journal of Pathophysiology
