摘要
目的 :探讨基质金属蛋白酶 7(matrixmetalloproteinase 7,MMP 7)与大肠癌浸润和转移的关系。 方法 :用逆转录聚合酶链式反应 (RT PCR)检测大肠癌和正常黏膜、炎性息肉、腺瘤及淋巴结中MMP 7mRNA的表达。结果 :97.4% ( 37/38)的大肠癌和 1例肝转移中MMP 7mRNA表达阳性 ,而相应正常黏膜几乎不表达 ;大肠癌中MMP 7mRNA的表达水平随Dukes分期的进展而逐渐升高 (P <0 .0 1) ,A期 ( 0 .2 8± 0 .0 8) ,B期 ( 0 .5 4± 0 .17) ,C期 ( 0 .5 5± 0 .0 9) ,D期 ( 0 .73± 0 .0 6 )。 5例腺瘤MMP 7均为阳性 ,但表达水平低于癌组织 (P <0 .0 1) ;3例炎性息肉均未见MMP 7表达。传统组织学检查有癌转移的 13个淋巴结MMP 7表达均为阳性 ;组织学检查未见转移的12个淋巴结MMP 7表达 6个阴性、6个阳性 ,再次行组织学检查于 6个MMP 7阳性者中发现 3个有微转移。结论 :MMP 7的高表达可能在大肠癌的浸润和转移过程中具有重要作用 ;用RT PCR方法检测淋巴结中MMP
Objective: To investigate the correlation of MMP 7 mRNA expression with the progression of colorectal carcinoma. Methods: RT PCR technique was used to measure the expression of MMP 7 mRNA in clinical specimens. Results: MMP 7 mRNA was observed in 97.4%(37/38)colorectal cancers and in 1 liver metastatic tumor, while it was almost undetectable in the normal mucosas. The level of MMP 7 mRNA increased in parallel with Dukes stage of colorectal carcinoma ( P <0.01); as: Dukes A, 0.28±0.08; Dukes B, 0.54±0.17; Dukes C, 0.55±0.09; Dukes D, 0.73±0.06. MMP 7 mRNA was also observed in 5 cases with colorectal adenomas, but its expression level was lower than that of colorectal carcinomas. All 13 histologically positive lymph nodes were positive for MMP 7 mRNA, while of 12 histologically negative lymph nodes, 6 were positive and the other 6 were negative. When histological re examination was performed on 12 histologically negative lymph nodes, micrometastases were detected in 3 MMP 7 positive lymph nodes. Conclusion: The overexpression of MMP 7 may play an important role in infiltration and metastasis of colorectal neoplasms. RT PCR assay for MMP 7 mRNA is a sensitive method for detecting lymph node micrometastases in patients with colorectal carcinoma.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2001年第1期13-16,共4页
Journal of Peking University:Health Sciences
关键词
大肠癌
基质金属蛋白酶-7
MRNA
肿瘤转移
Colonic neoplasms/enzymol
Metalloproteinases/metab
RNA, messenger/metab
Neoplasm metastasis
