摘要
利用PCR方法获得 116 3bp的戊型肝炎 (HepatitisEVirus,HEV)开放读码框架 (OpenReadingFrame ,ORF)ORF2之 3’大片段和 36 9bpORF3的完整片段 ,分别克隆到真核表达载体 pcDNA3中 ,构建两种含有HEV主要抗原表位的质粒DNA :pcE2和pcE3 ,分别或混合免疫Swiss小鼠三次 (0时 ,第 2周 ,第 4周 ) ,观察其在小鼠体内诱发的体液免疫应答。ELISA检测结果表明 ,pcE2和 pcE3在小鼠体内均可诱导出一定水平的HEVIgG抗体 ,且在第三次免疫接种两周后 ,10 0 %的小鼠抗体阳转。与两种质粒单独免疫相比 ,两者同时注射的抗体水平较高。本研究为HEVDNA疫苗的研究打下一定基础。
Two plasmid constructs, pcE2 and pcE3, containing 3' fragment of open reading frame 2 (ORF2,1163 bp) of hepatitis E virus (HEV) and full length ORF3 (369 bp), were injected into bilateral tibialis of Swiss mice respectively,for three times (0, 2 nd and 4 th weeks) and observed the HEV IgG by ELISA. HEV IgG was induced after the injection of pcE2 or pcE3 or both, and the percentage of seraconversion was 100% after two weeks of the third injection. Compared with injection of either construct, the antibody titers were higher in the group with combined injection of two constructs.
出处
《中国病毒学》
CSCD
2001年第2期131-134,共4页
Virologica Sinica
基金
云南省自然科学基金!资助项目 (97C0 79M )
