摘要
目的 探索促血小板生成素 (Thrombopoietin ,TPO)分子间相互作用及其存在位置。方法 构建了含有不同TPO区段的酵母双杂交系统融合表达质粒 ,转化酵母菌SFY5 2 6 ,分析 β 半乳糖苷酶活性以推测TPO分子间的相互作用。结果 全长TPO pGBT9与全长TPO pGAD42 4、全长TPO pGAD42 4与TPO(N) pGBT9、TPO(N) pGBT9与TPO(N) pGAD42 4之间有相互作用 ,全长TPO pGBT9与TPO(C) pGAD42 4、TPO(N) pGBT9与TPO(C) pGAD42 4、TPO(C) pGBT9与TPO(C) pGAD42 4之间无相互作用。结论 自然状态下TPO在N端结构区可能存在相互作用 ,C端结构域不参与此过程。
Objective To explore the interactions between TPO molecules and their locations.Methods Full length TPO,TPO(N) and TPO(C)were inserted respectively into the plasmids of the yeast two hybrid system.Six different recombinant plasmids were generated.Every 2 recombinant plasmids were transformed into the yeast cell SFY526.The possible interactions between TPOs were then tested by the bioactivity of β galactosidase.Results There are interactions between full length TPO themselves TPO(N)themselves and TPO with TPO(N),but not between TPO(C)themselves,TPO with TPO(C)and TPO(N)with TPO(C).Conclusion Naturally,the molecular interaction domain of TPO may exist in the N terminus but not in the C terminus.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2001年第2期125-127,共3页
Chinese Journal of Experimental and Clinical Virology
