摘要
大丽轮枝菌 (Verticiliumdahliaekleb .)是引起棉花黄萎病的病原真菌。使用从大丽轮枝菌V4 4 (高毒 )和V6 4 (低毒 )的菌丝体制备的诱导物 (I4 4 和I64 )作用悬浮培养的豫棉 6号 (感性 )和豫棉 8号 (耐性 )细胞系 ,用过氧化物酶法测定诱导后 30min内的反应性氧变化 ,发现仅有不亲和性较高的体系 ,即弱毒力的大丽轮枝菌 (V64 )和耐性的豫棉 8号所组成的体系 (I64 -Y8)表现最高的反应性氧迸发 ,3min - 6min时增加 6 1 8%。使用显著低于杀菌浓度的剂量的水杨酸 (SA)和H2 O2 作用上述大丽轮枝菌 ,发现水杨酸和H2 O2 都能影响微生物 ,经 1mmol/L水杨酸和 0 2mmol/LH2 O2 作用后的微生物 ,其所产生的诱导物对植物细胞反应性氧的诱导作用要高于未被作用的微生物的诱导物。两种化学性质的影响的共同点是使反应性氧迸发的峰值时间提前 ,峰值增加 ,水杨酸提前 12min ,H2 O2 的影响还突出表现在使反应性氧迸发的曲线锐化 ,即峰值时间范围的平均每分钟增加率表现显著增加 ,9min - 12min时达到 10 9%
Verticilium dahliae kleb is the pathogen which can induce the Verticillium wilt of cotton. The elicitors I 44 and I 64 can be prepared from mycelium of V. dahliae kleb. : V44(high virulence) and V64(low virulence). H 2O 2 can be detected during the first 30 minutes when the suspension-cultured cotton cells Y6(sensitive) and Y8(tolerant) had been induced by I 44 and I 64 .The highest oxidative burst can be detected in the higher incompatibility system I 64 -Y8,the increase rate is 61.8% during 3-6min. The elicitors from V. dahliae kleb which were treated by low concentration Salicylic acid(SA) (1mmol/L) and H 2O 2 (0.2mmol/L)can induce higher oxidative burst comparing with the control. Both of SA and H 2O 2 can ahead the peak of oxidative burst in the suspension-cultured cotton cells, about 6-12min,and increase the peak value, H 2O 2 also can sharpen the curve, notably increase the increasing rate to 109% around the peak value.
出处
《生物技术》
CAS
CSCD
2001年第4期17-19,共3页
Biotechnology
