摘要
为了寻找日本血吸虫病新的诊断和候选疫苗分子 ,设计合成引物 ,以日本血吸虫成虫cDNA第一链为模板 ,用PCR法扩增出日本血吸虫 14 - 3 - 3抗原 (Sj14 - 3 - 3 )基因编码序列 ,其大小约 784bp ,将其克隆入pGEM -T载体 ,重组质粒pGEM -T -Sj14 - 3 - 3经双酶切和以质粒为模板进行PCR扩增 ,均可获得一条与PCR产物一致的DNA片段 ,序列测定结果表明具有一个长度为 765bp的完整开放阅读框 ,与曼氏血吸虫 14 - 3 - 3核苷酸序列有高度同源性。本实验克隆了日本血吸虫 14 - 3 - 3抗原的编码基因 ,并进行了序列测定 ,为进一步研究提供了条件。
In order to discover new candidate vaccine molecular for schistosomiasis,primers were designed and synthesized The DNA encoding 14-3-3 antigen of S japonicum was amplified by polymerase chain reaction using SjcDNA as template The product from PCR was cloned into pGEM-T vector and sequenced The recombinant vector (pGEM-Sj14-3-3) was identified by restriction analysis and PCR For PCR, a specific band of around 784bp was amplified The same band was obtained by double restriction of recombinant plasmids and PCR using recombinant plasmids as template The nucleotide sequence has an open reading frame of 765bp It provided the basis for further study on Sj14-3-3 expression and its immunological diagnosis and prevention
出处
《中国人兽共患病杂志》
CSCD
北大核心
2001年第5期45-47,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金 (编号 395 70 64 6)
安徽省自然科学基金资助课题 (编号 97410 0 42 )
