摘要
为了研究作为三眠现诱导剂的一种唑化合物RK-42的作用机制，本文应用侧休(CA)短期体化学测定Bombyx motx则的活性，通过记蟆皮(MH)的垫射免疫分析(RIA)法测定了血苏巴内根据的放射免疫法成功珍KK-42作用后的肢导泌侃前胸蚊腺面的试验，提出了KK-42作用的新解说，吧调体活性的湖汇KK-42与侧体外支明KK-42应用下桑蚕四龄前期时，把器官首先见侧休，咽侧体看成性反使用分泌PTTH产生相应变化，从而引起蜕皮激素高峰的推迟。推迟了的蜕皮素峰与扣对馈管的保幼激素JH共同作用的结果引起了化蛹蜕皮，产生了三眠蚕。
In order to study the mechanism of action of the imidazole compound KK-42 which acts as an inducer of trimolter silkworm,the synthesis of juvenile hormone (JH) by isolated silkworm corpora allata (CA) was determined in vitro by means of a short-term radiochemical assay.The ecdysone liters in the haemolymph were determined accoirding to the method of radioimmunoassay.Combining the culture of the prothoracic glands (PG) and brains with the radioimmunoassay of ecdysone (MH),the secretory kinetics of PTTH in the brains after 'treatment with KK-42 was determined successfully.Together with other experiments,a new theory about the mechanism of KK-42 action is proposed.The result of the study on the synthetic activities of CA and the CA incubated with KK-42 in vitro indicated that the target organ of KK-42 was CA.When KK-42 was applied in the early stage of the fourth instar the synthetic activity of CA was decreased.Accordingly,it fedback to the brain so that the PTTH secretion was also decreased.The latter action delayed the increase of MH titer in the haemolymph.It was the co-action of this delayed MH peak and relatively lower JH titer that induced the precocious pupation and the trimoltism of the silkworm.
出处
《昆虫学报》
CAS
CSCD
北大核心
1991年第3期278-283,共6页
Acta Entomologica Sinica
关键词
桑蚕
保幼激素
内分泌
Bombyx mari----KK-42----juvenile hormone----ecdysone-PT TH----radioimmunoassay
