摘要
目的 观察去势后雄兔眼表及泪膜的变化 ,评价雄激素对泪液分泌及泪膜稳定性的影响。方法 将 16只雄兔随机分为去势 (实验 )及对照两组 ,每组 8只。实验组分别于去势前和去势后1、2、3、4周、2及 3个月行Shirmer试验、泪膜破裂时间 (break uptime ,BUT)及虎红染色检测 ;利用化学发光法检测实验组手术前、后血清睾酮水平 ,并行统计学分析处理。 3个月后处死两组兔 ,取其泪腺、Harder腺、结膜、角膜及角膜缘组织行病理组织学观察。结果 实验组Shirmer试验、泪膜破裂时间明显低于正常对照组 ,且随观察时间的延长差异有显著性 (t=9 0 32 ,P <0 0 1;t=4 747,P <0 0 1)。虎红染色 :实验组为阳性 ,对照组为阴性。实验组术后血清睾酮水平 (4 5 2± 0 81)nmol/L明显低于术前(19 76± 1 5 3)nmol/L(t=2 2 2 90 ,P <0 0 1)。病理学观察 ,实验兔泪腺上皮细胞胞浆萎缩扁平 ,腺腔扩大 ,腺泡泡状黏液消失 ,PAS染色阳性物质减少 ,结膜杯状细胞数量减少 ,角膜上皮及角膜缘干细胞形态学上无明显改变。结论 去势后雄兔睾酮水平明显低下 ,泪腺上皮细胞萎缩 ,腺泡泡状黏液物质消失及结膜杯状细胞减少 ,导致泪液分泌的质和量改变及泪膜稳定性降低。
Objective To evaluate the effect of androgen on tear secretion and tear film stability by observing the changes of ocular surface and tear film in the castrated male rabbit. Methods Sixteen male rabbits were selected and randomly divided into the normal control group (8 rabbits) and the castrated group (8 rabbits). Schirmer′s tests, tear film break-up time (BUT) and rose bangle (rb) staining were performed on the eyes of two groups at 1, 2, 3, 4 weeks, 2 and 3 months. The Chiron Diagnostics ACS:180 Automato Chemilluminescence Systems was used to determine the two groups′ testosterone level in serum at pre-castrate and post-castrate. Results were analyzed statistically. Lacrimal gland, Harder′s gland, conjunctiva, cornea and limbus were removed for observation histopathologically after three months. Results Both Schirmer′s test and break-up time scores were significantly lower in the castrated group compared with the control group. The difference became more and more prominent in the course of observation. Rose bangle staining was positive in the castrated group. Testosterone level in serum was significantly lower in the post-castrate than that in the pre-cartrate. Histopathological observation showed that lacrimal glandular epithelial cells were atrophic and flat, the lumen of gland was enlarged and vesicular mucous in acinus cells disappeared. PAS positive material and conjunctival globlet cells were significantly decreased in the castrated group. Compared with the control group there were no significant morphologic changes in the corneal epithelium and limbal stem cells. Conclusions The low level of testosterone in castrated rabbit may contribute to the atrophy and applanation of lacrimal glandular epithelium, disappearance of vesicular mucous in acinar cells and the significant decrease of the number of conjunctival globlet cells. All these can lead to decrease of both the quantity and quality of tear secretion and thus cause the instability of tear film.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2001年第6期458-461,T005,共5页
Chinese Journal of Ophthalmology
