摘要
目的 :超氧化物歧化酶 (SOD)的测活方法很多 ,目前国内多采用连苯三酚自氧化法 ,本方法有两种 ,一种是经典的Marklund连苯三酚自氧化法 ,简称 42 0nm法 ;另一种是对 42 0nm法作了改进的微量连苯三酚自氧化法 ,简称 32 5nm法。本文就这两种方法的特点及换算关系做了比较 ,希望可以改变SOD研制及应用中活力计算混乱的局面。方法 :在严格控制实验条件一致的情况下 ,对 2种方法对比考察 ,对所得数据进行线性回归统计。结果 :32 5nm与 42 0nm法间活力单位的比值为 1/ 2 5 ,且 32 5nm法的灵敏度高于 42 0nm法 。
Objective:There are many methods to test the activity of superoxide dismutase(SOD).In our country,the method of pyrogallol self-oxidation was used.Two kinds of this method,one is classical method known as 420 nm method);the other is improved micromethod known as 325 nm method).In this paper,the relation between the two methods of testing SOD activity was indicated.Method:Under identical conditions of test circumstances,the two kinds of method were compared.Result:In this paper,the conclusion is one enzyme unit of 325 nm method equals to 2 5 enzyme unit of 420 nm method.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2001年第5期328-331,共4页
Chinese Journal of Pharmaceutical Analysis
