摘要
目的 :建立人乳腺癌裸鼠移植模型 ,并探讨其部分生物学特性。方法 :采用雌激素受体阳性的 MCF- 7人乳腺癌细胞株 ,接种于 2 0只裸小鼠右侧胸壁乳垫下 ,移植细胞总数为 1× 10 6 /只。观察肿块生长情况 ,至 90天处死荷瘤鼠 ,切除肿块作病理切片 ,角蛋白19( KT19) m RNA、雌激素受体 ( ER)检测。结果 :接种后第 10天在接种部位可见结节 ,肿瘤移植成功率 (成瘤率 )为 95 %( 19/2 0 )。切除肿块平均直径为 ( 14± 3 ) mm,平均重量为 1.4 g,病理学检查为浸润性导管癌 ,RT- PCR检测表达人角蛋白 19( KT19)、ER阳性。结论 :该方法建立的人乳腺癌裸鼠移植模型 ,成功率高 ,肿瘤可部分保持人乳腺癌生物学特性 。
Objective:To establish a proper model of human brea st carcinoma in nude mice and study their biological features.Methods:MCF 7 cells(ER+) were implanted into the right breast pads of 20 nude mice. Tumour growth was observed, and the tumour bearing mice were kil led on day 90. The tumour masses were removed, and pathologically examined by th e microscope. Then human KT19 mRNA and ER protein were detected by RT PCR and i mmunohistochemistry respectively.Results:The tumour tubercles could be formed on day 10. The tumour ta ke rate was 95%(19/20),and average diameter and weight of the tumour were (14±3) mm and 1.4 g separatively. All of the specimens were infiltrating ductal carcinoma , and expressed human KT19 mRNA and ER protein.Conclusion:We succeeded in establishing human breast carcinoma model i n nude mice, which reserved certain biological features of human breast carcinoma, therefore it may be an available model for further research of breast carcinoma .
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第6期509-510,F003,共3页
Journal of Nanjing Medical University(Natural Sciences)
