摘要
目的 :探讨肾康注射液对肾小管上皮细胞LLC -PK1分泌层粘连蛋白 (LN)的影响。方法 :用细胞酶联免疫吸附 (ELISA)方法 ,以单味大黄注射液为实验对照组 ,检测肾康注射液对肾小管上皮细胞LLC -PK1分泌LN含量的影响。结果 :肾康注射液可以显著抑制肾小管上皮细胞LLC -PK1分泌LN的含量 ,并呈剂量依赖关系 ;肾康注射液作用明显优于同等含量的单味大黄注射液。结论 :肾康注射液抑制肾小管上皮细胞LLC -PK1分泌LN含量 ,是该方延缓慢性肾衰竭进展的机理之一。
Objective: To study the effect of Shenkang injection on the Laminin (LN) secretion in LLC-PK1 renal tubular epithelial cells. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to observed the effect of shenkang injection on LN production by LLC-PK1 renal tubular epithelial cells. The control group was treated with the injection extracted from Rheum officinale Baill. Results: Shenkang injection inhibited LN production by LLC-PK1 renal tubular epithelial cell more remarkable than the injection extracted from Rheum offcinale Baill. Conclusion: The mechanism of delaying chronic renal failure with Shenkang injection is achieved by inhibiting LN production by LLC-PK1 renal tubular epithelial cell.
出处
《中国中西医结合肾病杂志》
2001年第9期505-507,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
国家中医药管理局资助项目 (No 92B0 89)
并获国家中医药管理局科技进步一等奖 ( 92 -1-0 5 )
