摘要
引起小麦赤霉病的禾谷镰孢菌 (Fusarium graminearum)在华东地区对多菌灵已出现了高水平抗药性。本文报道了 F.graminearum对多菌灵的敏感性基线及其抗药性菌株生物学特性。离体条件下多菌灵对 10 0个野生敏感型菌株的平均 EC50 和 MIC值分别为 0 .5 74 8± 0 .0 133μg/ml和<1.4 μg/ml。而对 5 0个抗药性菌株的平均 EC50 和 MIC值则分别为 9.2 375 μg/ml和 >10 0 μg/ml。在麦穗上多菌灵对 5 0个敏感菌株和抗药性菌株防效的平均 EC50 分别为 2 82 .6μg/ml和 >2 0 0 0μg/ml。从田间获得的抗多菌灵菌株对苯菌灵、噻菌灵、甲基托布津表现交互抗药性 ,但不同于室内突变菌株 ,对乙霉威不表现负交互抗药性。抗药性菌株的无性和有性繁殖后代以及在无药培养基上菌丝体转代培养后 ,抗药水平保持不变。抗药性菌株的菌丝生长速率、产孢能力及致病力等与敏感菌株相比没有差异。
Resistance to carbendazim (MBC) has seriously developed in Fusarium grami nearum , which causes wheat scab in East of China. This paper presented the sensitivity base line of the pathogen to MBC fungicide and the biological character of the MBC resistant isolates. EC 50 and MIC values of carbendazim inhibiting mycelium growth of 100 wild type isolates were 0.5748±0.0133 μg/ml and less than 1.4 μg/ml in average, and those of 50 resistant isolates were 9.2375 μg/ml and more than 100 μg/ml, respectively in vitro . Average EC 50 of the fungicide controlling wheat scab was 282.6 μg/ml and more than 2 000 μg/ml on the detached ears which were respectively inoculated with 50 sensitive and resistant isolates. MBC resistant isolates showed positive cross resistance to benomyl, thiabendazole, thiophanate methyl, but no negative cross resistance to diethofencarb, which differed from lab resistant mutants. Resistance of F. graminearum to carbendazim is heritable through asexual and sexual cycles. No difference was found between the resistant and sensitive strains in the rate of mycelial linear growth, sporulation and pathogenicity.
出处
《植物病理学报》
CAS
CSCD
北大核心
2001年第4期365-370,共6页
Acta Phytopathologica Sinica
基金
国家"九五"科技攻关项目 ( 96- 0 0 5 - 0 1- 0 7)
江苏省"九五"科技攻关项目 ( BE993 12 )
高等学校骨干教师资助计划
关键词
小麦赤霉病
禾谷镰孢菌
多菌灵
抗药性菌株
生物学特性
Wheat scab
Fusarium graminearum
carbendazim
fungicide resistance
biological character
