摘要
利用含花粉特异性启动子Lat5 2的质粒pLat5 2 7、含细胞毒素A链基因的质粒pGA96 8以及表达质粒载体pGA987,进行酶切连接 ,构建了含有Lat5 2特异性启动子和细胞毒素A链基因的表达载体 .经酶切和PCR进行鉴定 ,证明所构建的重组载体即为实验所需的载体质粒 。
The plasmid pLat52\|7 which includes the anther\|specific promoter Lat52 is used to construct a expressed plasmid with plasmid pGA968 consisting DTA gene and pGA987 through the cleavage and linking.By the identification of enzyme and Lat52\|specific primer PCR,the vector we constructed is right and it makes the basis for the work in the future.
出处
《首都师范大学学报（自然科学版）》
2001年第4期62-67,共6页
Journal of Capital Normal University：Natural Science Edition
