摘要
目的 探讨nephrin在蛋白尿发生中的可能作用。方法 用免疫组化及图象分析的方法 ,分别对临床表现为大量蛋白尿 ,单纯性血尿患儿及对照组肾组织切片上nephrin的表达进行检测。结果 (1)大量蛋白尿组的nephrin表达量为 0 6 2± 0 2 4,与单纯性血尿组 (0 6 7± 0 2 3)及对照组(0 82± 0 17)比较三者之间差异无显著意义 (P >0 0 5 )。 (2 )大量蛋白尿伴弥漫性足突融合组nephrin表达量为 0 6 1± 0 2 5 ,与大量蛋白尿不伴弥漫足突融合组 (0 6 2± 0 2 5 )及对照组比较三者间差异无显著意义 (P >0 0 5 )。 (3)弥漫足突融合微小病变组nephrin表达量为 0 5 0± 0 15 ,与非微小病变 (0 6 5±0 2 7)及对照组比较差异无显著意义 (P >0 0 5 )。结论 在获得性肾小球疾病中 ,用免疫组化的方法未能检测到肾小球nephrin的表达变化。
Objective Nephrotic syndrome (NS) is one of the most common renal diseases in children. NS is characterized by heavy proteinuria clinically and the diffuse fusion of podocyte foot processes under the electron microscopy. The mechanisms of proteinuria are not clear. In 1998, Professor Tryggvason identified the first protein molecule of the slit diaphragm, nephrin. The defect of nephrin is the cause of Finnish type congenital nephrotic syndrome, while the change of nephrin in acquired renal diseases is unknown. In this study, we detected the expression of nephrin in glomerulus of patients with acquired renal diseases in order to disclose the possible effect of nephrin. Methods A total of 89 renal samples was included in this study, among which 73 cases were children with heavy proteinuria (the urinary protein exceeded 1 g/24), 16 cases were children with isolated hematuria. According to the findings of electron microscopy, children with heavy proteinuria were divided into the group with the fusion of foot processes and the group without the fusion of foot processes. Furthermore, children with the fusion of foot processes were also divided into groups with and without minimal change nephrosis. As controls, 4 normal tissues of nephrectomized kidneys from patients with kidney tumors were collected. Immunohistochemistry was performed on biopsy samples. The intensity of staining by immunohistochemistry was semiquantitated by image-analysis system. Results were corrected for reaction efficiency by the expression ratio of nephrin over carcino-embryo-antigen. Results (1) The expressions of nephrin in the group with heavy proteinuria (0.62±0.24) and isolated hematuria (0.67±0.23) showed no significant difference as compared to controls (0.82±0.17). (2) There was no significant difference in the expression of nephrin between groups with and without fusion of foot proceses (0.61±0.25 vs 0.62±0.25). (3) There were no significant differences in the expression of nephrin between groups with and without minimal change nephrosis (0.50±0.15 vs 0.65±0.27). Conclusion With immunohistochemistry, no major alteration in the expression of nephrin in acquired renal diseases was observed.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2001年第12期718-721,共4页
Chinese Journal of Pediatrics
基金
国家自然科学基金 ( 39970 775 )
