摘要
目的 探讨烫伤后创面组织中生长相关因子c fos、c myc和bFGF表达的变化规律以及c fos在伤后皮肤创面愈合过程中的调控作用 ,研究蛋白激酶C通路对上述因子表达的影响。 方法大鼠 188只 ,制成 30 %深Ⅱ度烫伤模型 ,随机分为正常对照组 (8只 )、单纯烫伤组、c fos抗体处理组、bFGF治疗组、蛋白激酶C通路抑制剂 (H7)组以及蛋白激酶C通路激活剂二乙酰一肟组 (各 36只 )。采用原位杂交、逆转录聚合酶链反应 (RT PCR)和免疫组织化学方法检测创面组织c fos、c myc与bFGF基因及蛋白表达规律。 结果 (1)烫伤诱导c fos表达增加在前 ,其次是bFGF ,最后是c myc ,分别于伤后 3h、1d以及 3d到达面密度峰值 (14 5± 1 3,t=13 30 6 ;0 15± 0 0 4,t=3 46 0和 0 11± 0 0 5 ,t=2 2 92 ;与单纯烫伤组比较均P <0 0 5 )。 (2 )应用外源性c fos抗体减少创面组织 3种因子的表达 ,其中c myc与bFGF基因表达量分别下降 80 %和 85 %。 (3)H7明显抑制c fos与bFGF基因及蛋白的表达 ,而使c myc基因及蛋白的表达高峰时间延迟至伤后 6h ;二乙酰一肟作用与H7相反 ,它可以使c myc表达高峰提前至伤后 6h ,同时使c fos与bFGF表达量分别增加至 0 2 3± 0 0 7(t=2 942 )和 0 6 9± 0 0 7(t=5 134,与单纯烫伤组比较均P <0 0
Objective To observe the time course and tissue distribution of c fos, c myc and basic fibroblast growth factor(bFGF) genes and their proteins in burned skin, and explore the possible network regulation mechanisms of c fos,c myc and bFGF in wound healing and the role of the protein kinase C pathway involved in signal transduction among these growth related factors. Methods The study was carried out on 188 Wistar rats. Animal model with 30% partial thickness scald injury was made and all rats were randomly divided into several groups: normal control ( n =8), injured control ( n =36), anti c fos ( n =36), bFGF ( n =36), 1 5 Isoquinolinyl sulfony 2methylpiperazine (H 7), and 2, 3 Butanedione monoxime (BDM) ( n =36). In situ hybridization, reverse transcription polymerase chain reaction (RT PCR) and streptavidin peroxidase (SP) immunohistochemistry methods were used to detect the gene and protein expression of c fos, c myc and bFGF in the wounded tissues. Results (1) The first appeared induction of growth related gene was c fos, the second bFGF, and the last c myc. They were peaked at 3 h,1 d and 3 d postburn with values of 14 5±1 3, t =13 306;0 15±0 04, t =3 460 and 0 11±0 05, t =2 292, respectively ( P <0 05). (2) Treatment with c fos antibody decreased the expression of these three growth related factors when compared with that in the controls. There were a 80% decrease in c myc and a 85% decrease in bFGF. (3) Treatment with H 7 inhibited the gene and protein expression of c fos and bFGF. The peak times of c myc gene and protein were delayed to 6 hours post injury. Treatment with BDM induced an earlier expression of c myc mRNA at 6 hours, and increased the gene and protein expressions of c fos and bFGF. The total amount of their values was increased to 0 23±0 07, t =2.942 and 0 69±0 07, t =5.134 ( P <0 01). Conclusions Thermal injury can induce a series of growth related gene expression showing a phasic control and regional distribution. c fos plays an important role in wound healing process. Protein kinase C pathway may repair wound.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2001年第9期714-717,共4页
Chinese Journal of Surgery
基金
国家重大基础研究规划资助项目(G19990 5 42 0 4)
国家杰出青年科学基金项目 ( 395 2 5 0 2 4)
国家自然科学基金资助项目 ( 39870 731)
