摘要
目的 选择猪骨作为rhBMP 2的载体 ,经去抗原处理 ,复合重组人工骨 ,观察该种人工骨临床应用骨缺损修复 ,促进骨折愈合及诱导成骨的能力。方法 选择猪骨经透析冻干法脱脂、脱蛋白及部分脱钙处理后与成品rhBMP 2按一定比例组成具有生物活性的猪骨 -rhBMP 2复合人工骨 ,先行动物模型实验 ,随后以自愿随机的原则 ,临床应用 32例 ,术后 1,4,8,12周进行X线检查 ,观察临床表现 ,功能恢复情况。结果 临床 32例 ,34块骨 ,35处植入。随访 31例 ,随访率 96 7% ,最长 2 4个月 ,最短3个月 ;骨的愈合及缺损修复满意 ,临床无不良反应。结论 经透析冻干法处理的猪骨能成为rhBMP 2的理想载体 ,与rhBMP 2复合后具有良好的成骨诱导活性 ,无免疫排斥反应 ,具有广泛的临床应用前景。
Objective Pig bones eliminated of the antigens were used for rhBMP-2 carriers to compound and reconstruct artificial bones.The ability of this artificial bone in repairing bone defects,promoting fracture healing and inducing bone formation were observed Methods Pig bones with the fat separated albumen eliminated together with parts of calcium by means of dialysis lyophilization and then composed biology active artificial pig bones-rhBMP-2 with rhBMP-2 were composed in a certain proportion.The bones were applied in animal model experimental studies and then used clinically in 32 cases at random based on voluntary principle.Clinical manifestation and function recovery were observed by X-rays examination in 1,4,8 and 12 weeks after operation.Results 32 cases of 34 bones with 35 insertion were used.96 7% of the 31 cases were followed up from 3～24 months.The bone repair and the patients recovery were satisfactory and there was no side reaction.Conclusion The processed pig bone is an ideal carriers of rhBMP-2 and has the action of inducing bone formation,without immunological rejection and it is of extensive prospects in clinical application.
出处
《中国骨伤》
CAS
2001年第12期719-721,共3页
China Journal of Orthopaedics and Traumatology
