摘要
-鹅膏毒(环)肽和二羟鬼笔毒(环)肽是剧毒的鹅膏菌和其它几种致死毒菌中由一些修饰氨基酸组成的环肽毒素。由于-鹅膏毒肽对真核生物的mRNA合成的专一性抑制和和二羟鬼笔毒肽对肌动蛋白的专一性束缚,因而它们在分子生物学和细胞学研究中具有重要应用,对其需求逐步增加。为此,作者使用了一种改良的毒素提取方法,以制备高效液相色谱从灰花纹鹅膏菌中分离制备-鹅膏毒肽和二羟鬼笔毒肽,并通过紫外吸收光谱和质谱进行鉴定,表明-鹅膏毒肽和二羟鬼笔毒肽的分离效果好,纯度高。本方法对其它毒菌中的-鹅膏毒肽和二羟鬼笔毒肽的分离制备具有同样的应用价值。
amanitin and phalloidin are cyclic peptide toxins composed of some modified amino acids distributing mainly in poisonous Amanita mushroom. The application of these toxic peptides in life science research is extending. In this report, an improved extraction method was employed andα-amanitin and phalloidin were prepared with reverse-HPLC. The isolated toxins were identified by UV absorbent spectra and mass spectra. It was showed that the isolated toxins were homogeneous. These procedures can be used for isolatingα-amanitin and phalloidin from other poisonous mushroom.
出处
《菌物系统》
CSCD
北大核心
2002年第1期112-115,共4页
Mycosystema
基金
国家自然科学基金资助项目93-(7)-03 and 96C-02-03-07] ~~
