摘要
目的 :对四川省石渠县鼠疫耶尔森菌的遗传特性进行分析 ,并与其他类型的鼠疫菌株相比较。方法 :特征性基因的PCR扩增、随机扩增多态性DNA、脉冲场电泳、rRNA基因指纹图分析等。结果 :石渠县的菌株为典型的鼠疫菌株 ,具有鼠疫强毒菌的典型特征。从该县青海田鼠中分离的鼠疫菌株 ,其分子生物学特征与我国其他疫源地中的鼠疫菌株明显不同。结论 :该地可能存在一种新类型的鼠疫自然疫源地 ,其鼠疫菌属于一单独型别 ;1999年该县发生的人间鼠疫 ,感染来自该疫源地之外 ;对该疫源地仍须密切监视 ,以确定其对人类的威胁。
Objective: Analyzing genetic characters of Yersinia pestis strains isolated from Shiqu County, Sichuan Province, China, and comparing with those of other types of Y. pestis in China. Methods: PCR of specific genes, Random Amplifying Polymorphism DNA, Pulse-field gel electrophoresis, and rRNA gene fingerprinting. Results: The results showed that Shiqu strains were typical Y. pestis strains with all the tested virulence and other specific genes. The strains from Microtus fuscus, however, had unique characters, significantly different from the strains isolated from other parts of China. Conclusion:The results suggested that a new type of plague natural focus existed in that area; The human outbreak of 1999 was infected outside of those natural foci; The focus should be put under close surveillance, and its risk to human beings has to be determined.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2002年第1期48-52,共5页
Chinese Journal of Vector Biology and Control
