摘要
目的 :探讨格林巴利综合征 (GBS)是否存在中枢神经系统脱髓鞘现象。方法 :应用酸提法从胎儿大脑白质中提取髓磷脂碱性蛋白 (MBP) ,经Sephadex G15 0柱层析纯化和SDS PAGE圆盘电泳分析 ,以纯化的MBP为抗原 ,用ELISA法测了 4 4例GBS患者、38例其他神经疾病 (OND)患者和 33例正常人。结果 :经酸提后的碱蛋白 ,共有 3个洗脱峰 ;SDS PAGE证实第三峰中含 1.7kD和 2 .1kD两条区带 ;GSF MBP EgG检测 ,GBS组阳性率为 38.6 % (17/ 4 4 ) ,OND组阳性率为 34% (13/ 38) ,对照组阳性率为 9% (3/33)。结论 :MBP作为髓鞘的主要蛋白在GBS的自身免疫性脱髓鞘的过程中起重要作用。
Objective:To explore whether demyelination exists in central nervous system in GBS.Methods:MBP was isolated from white matter of fetal cerebrum by acid extration,purified by sephadex-G150 Chromatography and analysed by SDS-PAGE disc electrophoresis.With purified MBP as antigen,44 GBS patients,38 cases of othere neural diseases(OND) and 33 normal subjects were examined by ELISA assay.Results:There were 3 peaks in purified myelin basic protein.SDS-PAGE confirmed that two bands of 1.7 kD and 2.1kD were in the third peak.In CSF-MBP-IgG assay,the positive rates were 38.6%(17/44)in the GBS group,34%(13/38)in the OND group,9%(3/33)in the control group.Conclusion:MBP is one of the major protein of neural myelin and may play an important role in autiommune myelinoclasis.
出处
《山东医科大学学报》
2001年第5期410-412,共3页
Acta Academiae Medicinae Shandong
基金
山东省自然科学基金资助课题 ( 89c1332 )
