摘要
对He -Ne激光辐照天蚕卵后的当代蛹进行RAPD分析结果显示 :独特带型增多 ,遗传差距增大 ,其DNA多态性与GC含量有关。推测He -Ne激光辐照的天蚕卵后 ,可能容易诱发碱基转换 ,造成模板GC含量减少 ,与引物结合位点增多 ,从而导致RAPD扩增带的多态性增加。因此采用RAPD技术 ,可以从当代DNA分子水平的变化检测诱变效率。
Pupal DNA of Antheraea yamamai which had been irradiated by using He Ne laser at the period of egg was analyzed by RAPD. The results were that special bands increased,genetic distance became wider and DNA polymorphisms were related with GC content. Which suggested that the bases converted easily and GC content of template DNA reduced after induced by He Ne laser,resulting in DNA polymorphisms increasing for more locations combined with primers. Thus inducing efficiency can be checked by RAPD at the level of DNA molecular.
出处
《激光生物学报》
CAS
CSCD
2002年第1期23-27,共5页
Acta Laser Biology Sinica
基金
西南农业大学农业部蚕桑学重点实验室研究基金资助
