摘要
目的 :探讨 p5 3蛋白与肺癌多药耐药 (MDR)的关系。方法 :运用 S- P免疫组化法和反转录 -聚合酶链反应 (RT- PCR)技术 ,对 40例肺癌组织中 p5 3蛋白、多药耐药 (mdr- 1)基因和多药耐药相关蛋白 (mrp)基因表达进行检测。结果 :癌组织 p5 3阳性的 mdr- 1、m rp阳性率高于 p5 3阴性者 (P<0 .0 5 ) ;p5 3和 mdr- 1、m rp阳性率与肺癌的病理分期、组织学类型、分化程度和淋巴结转移无关 (P>0 .0 5 ) ,但它们在低分化肿瘤和淋巴结转移标本的阳性率高于中高分化和无淋巴结转移者。结论 :p5 3蛋白对肺癌细胞的 mdr- 1、mrp表达可能起调控作用 ,促使肺癌细胞 MDR的产生。
Objective:To investigate the correlation between p53 protein and multidrug resistance in lung cancer.Methods:The expression of p53 protein was examined by means of SP immunohistochemical technique,mdr 1 and mrp gene expression was determined by RT PCR method in 40 cases of lung cancer tissues proved by pathology and normal lung tissues of adjacent tumor.The relationship between the expression of p53 protein and mdr 1,mrp gene was analyzed respectively.Results:The positive incidence of mutant p53 was 65 %(26/40).In 26 cases of p53 positive expression,positive rates of mdr 1 and mrp gene was higher than that in p53 negative ( P <0.05).The positive expression of p53 protein and mdr 1 and mrp gene had no statistical significance in each TNM stage,pathological type,differentiated grades and with or without lymph node metastasis ( P >0.05),but their positive rates in low differentiation tumor and in patients with lymph node metastasis was higher than that of moderate to high differentiation carcinoma and in the cases without lymphatic metastasis.Conclusion:The positive correlative changes of mutant p53,mdr 1 and mrp supports the phenomenon of regulating effect of p53 on mdr 1 and mrp expression.
出处
《肿瘤研究与临床》
CAS
2002年第1期18-20,共3页
Cancer Research and Clinic
