摘要
室内离体叶片快速检测法结果发现,KMD1和KMD2两个转Bt抗虫基因纯合品系与常规感虫水稻品种杂交F1群体人工接虫2d后的幼虫死亡率高达100％,表明这两个品系的抗虫性由显性基因控制。田间调查结果发现,KMD1和KMD2与常规感虫粳稻品种杂交F2群体中抗虫株与感虫株的分离比符合3:1,BC1群体中抗虫株与感虫株的分离比符合1:1,表明这些纯合品系的抗虫性由一对显性核主基因控制。
It was found that newly hatched SSB larvae died 100.0% on the second days after being fed on leaf tissues of F1 population derived from the crosses between KMD1, KMD2 and conventional rice varieties by using cut leaf feeding method. This indicated that the resistance of Bt rice to SSB is conditioned by a dominant gene. Field evaluation demonstrated that Mendelan segregation for resistance to SSB in F2 and BC1 progenies from the crosses of Bt transgenic rice KMD1 or KMD2 with conventional rice varieties. This demonstrated that the resistance of Bt rice to SSB is conditioned by a major dominant gene.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第1期29-32,共4页
Scientia Agricultura Sinica
基金
浙江省科委"九五"重大项目(991102004-002)
美国洛氏基金资助项目(RF97001#556)
国家植物转基因专项(J2000B-009)
