摘要
目的 :探讨中药单体淫羊藿甙 (ICA)抑制肿瘤HL 6 0细胞端粒酶活性及作用机制。方法 :采用MTT法 ,NBT染色法 ,TRAP PCR ,RT PCR法和流式细胞术。结果 :ICA显著抑制HL 6 0细胞端粒酶活性 ,且端粒酶活性下降与细胞表面粒细胞分化抗原CD11b表达率呈负相关 ;诱导HL 6 0细胞向粒细胞方向分化 ;改变HL 6 0细胞周期各时相的分布 ,表现为G0 /G1期细胞逐渐增多 ,S期细胞逐渐减少 ;上调分化相关基因p2 1、下调增殖相关基因c mycmRNA和蛋白表达水平。结论 :阐明了ICA显著抑制HL 6 0细胞端粒酶活性 ,并从基因 蛋白 细胞效应水平揭示了其调节端粒酶活性的可能机制。
Objective:To study inhibited tecomerase activity of HL-60 cells in human cancer cell by Icarrin(ICA) and its mechanism.Methods:MTT assay,NBT assay, TRAP-PCR,RT-PCR assay,Flow Cytometry.Results:ICA inhibited telomerase activity in HL-60 cells significantly.It was negative correlation between telomerase activity and the expression ratio of CD11b antigen in HL-60 cells.It can induced HL-60 cells to differentiate into mature granulocytes.It can changed cell cycle distribution of HL-60 cells,which was reflected by the accumulation of the vast majority of cells in the G0/G1phase and the loss of cells in the S phase. It can downregulated cell proliferation related gene c-myc;upregulated p21 gene protein and mRNA expression.Conclusion:This study proved mechanism of ICA on inhibited telomerase activity from gene-protein-effect of cell level in HL-60 cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2002年第3期191-194,196,共5页
Chinese Journal of Immunology
基金
山东省科委资助项目
编号 9712 2 6 2 0 6
