摘要
目的 观察增殖细胞核抗原 (PCNA)反义寡核苷酸对人肝癌细胞株体外增殖的影响及生物学特性的改变。方法 MTT法、集落形成试验观察 PCNA反义寡核苷酸对人肝癌细胞的生长抑制 ,免疫组化技术检测 PCNA的蛋白表达 ,流式细胞仪分析细胞周期变化。结果 PCNA反义核酸作用后的肝癌细胞生长明显受到抑制 ,抑制效应于作用后 48h最为明显 ,并于 72 h后逐渐减弱。集落形成率明显下降。 PCNA蛋白表达明显下降。细胞周期中 S期细胞数明显减少。结论
Objective To investigate the inhibitory effect of PCNA antisense oligonucleotides on hepatocellular carcinoma cell proliferation.Methods The inhibition of hepatocellular carcinoma cell proliferation was estimated by MTT.The protein expression was analysed by immunohistochemistry S P method.The cell cycle was analysed by flow cytometric(FCM).Results The cell proliferation was inhibited effectively by antisense oligonucleotides.The cell growth rate was reduced nearly 50% after 48 hours treatment.The proportion of S phase was reduced.The inhibit effect discreased after 72 hours.Conclusion PCNA antisense oligonucleotides exerts a strong inhibitory effect on hepatocellular carcinoma cell proliferation.
出处
《肝胆外科杂志》
2002年第1期65-68,共4页
Journal of Hepatobiliary Surgery
基金
福建省教委科研基金项目 ( K2 0 0 6 3)
关键词
反义寡核苷酸类
增殖细胞核抗原
肝癌
细胞增殖
Oligodeoxynucleotides
Antisense
Liver neoplasm
Proliferation cell nuclear antigen
