摘要
抗生素长期滥用导致了人体内菌群失调及细菌耐药性的产生,因此需要寻找新型、靶向抗菌方法来治疗耐药细菌的感染。近年来,CRISPR/Cas系统的深入研究为设计特异性靶向耐药基因,定向清除耐药细菌的药物提供了新的思路。在此介绍了CRISPR/Cas系统作为新型抗菌方法,通过靶向切割抗性质粒或细菌基因组以实现对耐药基因或病原菌的特异性清除,并对CRISPR抗菌药的不同类型核酸酶的选择,以及CRISPR递送系统的运载工具进行了评价。
Antibiotics are used to arrest essential bacterial signaling and/or metabolic pathways,causing bacterial cell death. Overuse and misuse of antibiotics have led to dysbacteriosis and drug resistance. The application of CRISPR( clustered regularly interspaced short palindromic repeats)/Cas( CRISPR associated)systems provides a new method to kill the drug-resistant microbes specifically by designing programmable sequence-specific antimicrobials. Currently,the most efficient CRISPR/Cas systems are type Ⅰ and type Ⅱ as a novel antimicrobial tool in selective removal of bacterial pathogens. Bacteriophage has been developed as a delivery vehicle,but the membrane vesicles had more potential for transporting the CRISPR/Cas systems into the targeted resistant pathogen.
作者
曾家伟
侯国锋
郑继平
杨诺
曾纪峰
郭桂英
ZENG Jia-wei;HOU Guo-feng;ZHENG Ji-ping;YANG Nou;ZENG Ji-feng;GUO Gui-ying(Hainan University,Tropical Agriculture and Forestry College,Haikou 570228,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2018年第11期59-65,共7页
China Biotechnology
基金
国家自然科学基金(31460699)
海南自然科学基金创新研究团队项目(2017CXTD005)
海南自然科学基金面上项目(317071)资助项目
