摘要
目的研究采后水分及营养胁迫对黄芩中活性物质积累及其相关基因表达的影响,为揭示采后环节对药材品质及道地性形成的分子机制奠定基础。方法采用HPLC测定黄芩中活性物质(野黄芩苷、黄芩苷、黄芩素、汉黄芩苷、汉黄芩素)含量;采用Tiangen多糖多酚植物总RNA提取试剂盒提取RNA,采用TAKARA反转录试剂盒合成c DNA。结果采后水分及营养胁迫对黄芩中活性物质积累呈现促进效应,黄芩苷、汉黄芩苷、野黄芩苷、汉黄芩素及黄酮类总含量于采后0. 5 d-1 d出现明显提升,PAL、CHS、CHI、UBGAT与GUS基因均显著上调表达且野黄芩苷含量与UBGAT呈现显著正相关,相关系数为0. 748(P <0. 05)。结论采后水分及营养胁迫下黄芩启动抗逆机制,调节相关基因表达从而对次生代谢产物含量产生影响,暗示环境对药材品质及道地性的形成具有重要作用。
Objective In order to lay foundation for revealing the molecular mechanism of the quality of medicinal materials and the formation of geo nature,the effect of water stress and nutrition stress on the accumulation of effective components and expression of related genes in harvested Scutellaria baicalensis Georgi were studied in this paper. Methods The content of effective components(baicalin,baicalein,wogonoside,and wogonin) were detected by HPLC. RNA was extracted with RNA pre pure RNA purification kit(Tiangen) and TAKARA Reverse Transcription Kit were used to synthesis c DNA. Results The results showed that the water and nutrition stress promoted the accumulation of active components in Scutellaria baicalensis,the content of baicalin,baicalein,wogonoside,and wogonin were obviously evaluated in 0. 5 d and 1 d. The expression of PAL,CHS,CHI,UBGAT and GUS were up-regulated,moreover,the content of scutellarin and expression of UBGAT showed significant positive correlation,the correlation coefficient is 0. 748(P < 0. 05). Conclusion According to the results,the stress response of Scutellaria baicalensis were arose by regulating the expression of relative genes and then influenced the content of active components,which hinted environment plays an important role in the formation and quality of Scutellaria baicalensis.
作者
周冰谦
周洁
董红敬
耿岩玲
王晓
刘伟
ZHOU Bing -qian;ZHOU Jie;DONG Hong -jing;GENG Yan-ling;WANG Xiao;LIU Wei(Qilu University of Technology (Shandong Academy of Sciences ,Shandong Analysis and Test Center,Shandong Key Laboratory of TCM Quality Control Technology,Ji'nan 250014,China;School of Biological Science and Technology,University of Ji'nan,Ji'nan 250012,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2018年第10期2546-2550,共5页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(81803662):国家重点研发计划项目(2017YFC1700703):现代农业产业药术体系建设专项资金资助(CARS-21)国家中药材产业技术体系(CARS-21)
山东省自然科学基金(ZR2017BH018
ZR2017LH069):山东省重点研发计划(2018RSF116017).
关键词
黄芩
水分胁迫
营养胁迫
活性物质
基因表达
Scutellaria baicalensis
Water stress Nutrition stress
Effective component
Gene expression
